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Plasma cells were isolated from a mouse spleen cell suspension by using the CD138 + Plasma Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with CD45R (B220)-APC, CD19-FITC, and CD138-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated fraction | Pre-enriched plasma cells after depletion of CD49b (DX5) + cells and CD45R (B220) + cells |
CD138 + Plasma Cell Isolation Kit | CD138 + Plasma Cell Isolation Kit |
Isolated CD138 + plasma cells | |
CD138 + Plasma Cell Isolation Kit |
Plasma cells were isolated from a mouse spleen cell suspension by using the CD138 + Plasma Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with CD45R (B220)-APC, CD19-FITC, and CD138-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Unseparated fraction | Pre-enriched plasma cells after depletion of CD49b (DX5) + cells and CD45R (B220) + cells |
CD138 + Plasma Cell Isolation Kit | CD138 + Plasma Cell Isolation Kit |
Isolated CD138 + plasma cells | |
CD138 + Plasma Cell Isolation Kit |
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