Clone:
REA373
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC, MC
Alternative names:
BCL6, BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Extended validation for Bcl-6 Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA373
BCL-DWN-
K112-91-
7D-1++
IG-191E/A8-
BCL-UP-
Cells were incubated with an excess of purified unconjugated Bcl-6 (REA373) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for Bcl-6 Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA373 recognizes the human and mouse Bcl-6 antigen, a member of the BTB-zinc finger family of proteins. Bcl-6 is a transcriptional repressor, expressed in germinal center T and B cells and in primary immature dendritic cells. It plays critical functions in various cell types within the adaptive and innate compartments of the immune system. Bcl-6 is highly up-regulated in B cells after T cell–dependent antigenic challenge, and is required for formation of germinal centers within which B cells undergo immunoglobulin affinity maturation. It also plays an important role in macrophages, where it mediates a dampening effect on inflammatory signaling through repression of chemokine expression and NF-κB target genes.
Additional information: Clone REA373 displays negligible binding to Fc receptors.

Alternative names

BCL6, BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Detailed product information

Technical specifications

CloneREA373
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenBcl-6
Alternative names of antigenBCL6, BCL5, BCL6A, LAZ3, ZBTB27, ZNF51
Molecular mass of antigen [kDa]79
Distribution of antigenB cells, T cells
Entrez Gene ID604, 12053
RRIDAB_2801712, AB_2801827, AB_2801823, AB_2819665, AB_2801714

Resources for Bcl-6 Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Bcl-6 Antibody, anti-human/mouse, REAfinity™

Publications

  1. Shaffer, A. L. et al. (1997)
    In vivo
    occupancy of the kappa light chain enhancers in primary pro- and pre-B cells: a model for kappa locus activation.
    Immunity 6: 131-143
  2. Huang, C. et al. (2000) Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat. Immunol. 14(4): 380-388
  3. Kerckaert, J. P. et al. (1993) LAZ3, a novel zinc-finger encoding gene, is disrupted by recurring chromosome 3q27 translocations in human lymphomas. Nat. Genet. 5(1): 66-70

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