Immunofluorescence analysis of a formalin-fixed, paraffin embedded (FFPE) human breast cancer. Before staining, antigens were retrieved using heat-induced epitope retrieval (HIER) at pH 9. The section was stained using Actin (Smooth Muscle) Antibody, anti-human, FITC (green) for 10 min at room temperature (A). Actin (Smooth Muscle) Antibody, anti-human, FITC staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.

Immunofluorescence analysis of a human breast cancer cryosection fixed with 4% PFA. The section was stained using Actin (Smooth Muscle) Antibody, anti-human, FITC (green) for 10 min at room temperature (A). Actin (Smooth Muscle) Antibody, anti-human, FITC staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue). Scale bar: 30 µm.

Immunofluorescence analysis of a human breast cancer cryosection fixed with ice-cold acetone. The section was stained using Actin (Smooth Muscle) Antibody, anti-human, FITC (green) for 10 min at room temperature (A). Actin (Smooth Muscle) Antibody, anti-human, FITC staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue). Scale bar: 30 µm.

Immunofluorescence analysis of a mouse small intestine cryosection fixed with 4% PFA. The section was stained using Actin (Smooth Muscle) Antibody, anti-human, FITC (green) for 10 min at room temperature. Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.