MBTP 60 = Miltenyi Biotec-tested panel 60
This application protocol describes the determination of the cell fitness of chimeric antigen receptor (CAR) T cell products using Annexin V and 7-AAD for discrimination between viable, apoptotic, and dead cells. To demonstrate this state-of-the-art panel for CAR T cell fitness, CAR T cells were generated within 12 days using the CliniMACS Prodigy® T Cell Transduction process.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
7-AAD* | - | Viability | - | 655-730 (blue) |
Annexin V | REA1165 | Apoptosis | FITC | 525/50 (blue) |
CD4* | REA623 | Helper T cells | VioGreen™ | 525/50 (violet) |
CD8* | REA734 | Cytotoxic T cells | APC-Vio® 770 | 750 LP (red) |
CD3 | REA613 | T cells | APC | 655-730 (red) |
CD19 CAR FMC63 | REA1297 | CAR+ cells | PE | 585/40 (blue) |
* Alternative CE-IVD/ASR reagents available.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
7-AAD* | - | Viability | - | 655-730 (blue) |
Annexin V | REA1165 | Apoptosis | FITC | 525/50 (blue) |
CD4* | REA623 | Helper T cells | VioGreen™ | 525/50 (violet) |
CD8* | REA734 | Cytotoxic T cells | APC-Vio® 770 | 750 LP (red) |
CD3 | REA613 | T cells | APC | 655-730 (red) |
CD19 CAR FMC63 | REA1297 | CAR+ cells | PE | 585/40 (blue) |
* Alternative CE-IVD/ASR reagents available.
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