Reliable analysis of rare virus-specific T cells 

T cells play a pivotal role in fighting viral infections and generating long-term protective immunity after infection and vaccination. 

Assessment of specificity, phenotype, and cytokine secretion of rare virus-reactive T cells can provide essential information prior infections, on infection progression,  or on vaccination efficacy. 

Product highlight

SARS-CoV-2 T Cell Analysis Kits

All-in-one SARS-CoV-2 T Cell Analysis Kits to monitor and analyze human SARS-CoV-2–reactive T cell subsets from PBMC or whole blood samples. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator® Peptide Pool of choice and an optimized flow cytometry panel.

SARS-CoV-2 T Cell Analysis Kits
 

A complete workflow for stimulation, enrichment, and analysis of virus-reactive T cells

Isolation of PBMCs or T cells from human whole blood

Choose between our solutions for manual or automated cell isolation from human whole blood.  

Virus-specific in vitro stimulation of T cells and other immune cells 

We offer PepTivator® Peptide Pools for efficient antigen-specific stimulation of both CD4+ and CD8+ T cells, as well as recombinant SARS-CoV-2 proteins for stimulation of other immune cells. 

Application note
Fast and efficient detection of SARS-CoV-2–reactive T cells via antigen-specific stimulation

See how we used our new SARS-CoV-2 T Cell Analysis Kit (PBMC), human for the stimulation and analysis of virus-reactive T cells in the context of COVID-19.

Magnetic enrichment of antigen- and virus-specific T cells

Antigen- and virus-specific T cells are extremely rare, which makes analysis difficult. Our optimized enrichment solutions enable you to analyze even the rarest cells.

Protocol
Rapid antigen-specific T cell enrichment (Rapid ARTE)

Combine magnetic cell enrichment and multiparameter flow cytometric analysis for direct ex vivo characterization of human antigen-specific CD154+CD4+ T cells.

Safe flow sorting of antigen- and virus-specific T cells

When working with contaminated or infectious material, operator safety is of utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of rare virus-specific T cells in a closed aerosol-free cartridge system.

Scientific poster
Detection of humoral and cellular responses to SARS-CoV-2 in COVID-19 convalescents

Download our scientific poster to see how the MACSQuant Tyto can be used for sorting of SARS-CoV-2–specific B cells with fluorescent SARS-COV-2 antigen-tetramers. 

Multiparameter flow analysis of antigen- and virus-specific T cells

Our MACS® Flow Cytometry Kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility, and most precise results. The MACSQuant Analyzers with automated MACSExpress® Analysis Modes eliminate operator variability and standardize your analysis further.

  • SARS-CoV-2 T Cell Analysis Kits are true all-in-one kits for the efficient stimulation and analysis of human SARS-CoV-2–reactive T cells. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator Peptide Pool of choice and an optimized flow panel (see table below).
  • REAfinity™ Recombinant Antibodies are recombinantly engineered for higher purity, superior lot-to-lot consistency, and lowest background.
  • MACSplex Cytokine Kits for a multiplexed analysis of secreted cytokines in cell culture supernatant.
  • Rapid Cytokine Inspector Kits are unique kits that offer the fastest combination of surface marker and intracellular cytokine staining.
  • 8-Color Immunophenotyping Kit, human for reliable identification of leukocyte subsets in one simple kit, e.g., monocytes, neutrophils, eosinophils, and T, B, and NK cell populations.
  • StainExpress™ Immune Cell Composition Cocktail, human to achieve highest standardization. These dried antibody cocktails enable reliable evaluation of immune cell composition from human blood material (e.g. whole blood or PBMCs).
  • MACSQuant® Analyzers are fully automated benchtop flow cytometers for highly sensitive multiparameter flow analyses.

Video
Discover the beauty of automated flow cytometry 

All you have to do is choose your cells and markers of interest: Our family of MACSQuant Analyzers for different applications can handle the rest. 

Optimized flow panel for SARS-CoV-2 T Cell Analysis

MarkerCloneFluorophore 
CD3REA613 APC 
CD4REA623 Vio® Bright B515
CD8REA734 VioGreen™
CD154REA238APC-Vio® 770
IFN-g REA600PE 
IL-2REA689 PE-Vio® 615
TNF-a REA656PE-Vio® 770
CD14REA599 VioBlue®
CD20REA780 VioBlue 
Live/dead Viobility™ Fixable Dye 405/452 

Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.*

For Vio Dye spectra see here

Check out more supporting materials on antigen-specific T cells

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