Induced pluripotent and embryonic stem cell research workflow for culture and maintenance

StemMACS PSC-Brew XF, human

Ensuring a state-of-the-art PSC culture with stable doubling times, our products provide high expression of pluripotency markers, full pluripotent potential, and a stable karyotype. Our solutions enable hPSCs grown in StemMACS PSC-Brew XF, human the ability to show typical morphology, expression of pluripotency-associated markers and retain the ability to differentiate into cell types that derive from the three embryonic germ layers, even after consecutive passages in culture.

Functional assessment of pluripotency in just seven days

PSCs by definition, need to be able to differentiate into cellular types that derive from the 3 embryonic germ layers (endoderm, mesoderm, ectoderm). This property needs to be routinely assessed, to prove the highest quality of the line. To make this task easier and even more accurate, we have developed a standardized and quantifiable differentiation assay based on lineage-specific complete media that directly supports 2D differentiation into all three germ layers within seven days. 

The StemMACS Trilineage Differentiation Kit, human contains ready-to-use media to ensure reproducibility and minimizes your effort allowing a definitive side-by-side comparison of different cell lines or clones. 

Optimal storage and cryopreservation is essential for the stability and accuracy of many cell culture workflows. The importance of trusting such a medium to maintain these characteristics is vital in workflow success.  StemMACS Cryo-Brew is a cryopreservation medium that has been designed for use with xeno- and serum- free culture systems ensuring high recovery, viability, and genetic stability of PSCs.