Isolation of target cells increases the resolution of bulk and single-cell genomic analysis, by excluding the background signals coming from non-target cells, and thereby helps to save time and decrease sequencing costs. MACS® Technology allows the fast and easy immunomagnetic isolation of single cells from solid tissues and blood samples, helping to prevent changes in gene expression for more reliable and high resolution genomic analysis. MACS Technology also includes automated solutions to upscale, standardize, and speed up your magnetic cell separations for reproducible results.
Gentle direct isolation of PBMCs from whole blood using immunomagnetic beads prevents activation of cellular stress responses compared to density gradient centrifugation methods. In addition to that, the automated PBMC isolation procedure is standardized and reproducible, improving purity of the obtained PBMCs population.
Highly pure PBMCs directly isolated from whole blood using the Whole Blood PBMC Isolation Kit, human and Sedimentation Kit 2*
|Sample 1||Sample 2||Sample 3|
|Granulocytes||Whole Blood PBMC Isolation Kit||0,27%||0,29%||0,2%|
|Erythrocytes||Whole Blood PBMC Isolation Kit||0,12%||0,06%||0,06%|
* Please contact us for product and ordering information.
Magnetic isolation improves the resolution of single-cell RNA sequencing of T and B cell receptors, highlighting clonotypes otherwise poorly or altogether unrepresented in the bulk sample.
Magnetic isolation of neurons after dissociation of adult mouse brain substantially increases the resolution of single-cell gene expression profiling, allowing the identification of several neuronal subpopulations which otherwise could not be distinguished.
Tumor cell isolation enables accurate detection of single nucleotide polymorphisms (SNP) in solid tumors for the analysis of loss of heterozygosity using next generation sequencing (NGS).
Increased sensitivity of NGS-based mutation analysis of FFPE lung adenocarcinoma specimens by carcinoma cell enrichment.
|Specimen ID||Pre-processing prior DNA extraction||Concentration of extracted DNA (ng/ul)||DNA input for NGS||Driver mutation identified||Coverage||Allele frequency|
|Specimen 1 (70% tumor cells)||Macrodissection||48.8||40||EGFR: c.2236_2250del;|
|Enriched tumor cells||38.8||40||EGFR: c.2236_2250del;|
|Specimen 2 (10% tumor cells)||Macrodissection||4.78||20||KRAS: c.34G>T; p.G12C||388||11%|
|Enriched tumor cells||0.46||20||KRAS: c.34G>T; p.G12C||623||28%|
|Specimen 3 (< 5% tumor cells)||Macrodissection||5.64||40||KRAS: c.34G>T; p.G12C||405||1%|
|Enriched tumor cells||2.12||40||KRAS: c.34G>T; p.G12C||951||7%|
MACS Technology enables the magnetic separation of cell populations by targeting surface antigens with specific antibodies that are conjugated to superparamagnetic beads. Labeled cells are magnetically retained in a separation column, from which they can easily be eluted. The fast and gentle isolation minimizes cellular changes and ensures high viability. Explore the different solutions for target cell isolation with MACS Technology here.
Whether working with only few samples or large sample cohorts, automation helps to streamline cell separation. The MACS Cell Separation instruments allow different levels of automation according to the sample throughputs. In particular, the autoMACS Pro Separator provides full automation for magnetic cell separations using MACS Technology, while minimizing temperature changes and user-dependent variation.
Pre-enrichment of target cells reduces the flow acquisition time
|Cell type||Cells to analyze||Events to collect||Flow cytometry time/sample*||Total flow cytometry time**|
|CD4+ T cells|
|Bulk||5,000||7.96×10⁶||66.3 min||>10 h|
|Isolated***||5,000||5.41×10⁴||0.5 min||~11 min|
|CD8+ T cells|
|Bulk||5000||2.80×10⁶||23.3 min||>3.5 h|
|Isolated***||5000||4.37×10⁴||0.4 min||~10 min|
|Bulk||10,000||8.13×10⁵||6.8 min||>1 h|
|Isolated***||10,000||3.24×10⁴||0.3 min||<10 min|
|* Flow rate: 2,000 events/s|
** Considering 9 samples (3 experimental groups × 3 replicas/group). Includes 45 s automated mixing and rinsing between samples on the MACSQuant® Instrument
*** Isolation using CD8 (TIL), CD4 (TIL), or CD4/CD8 (TIL) MicroBeads, respectively
Isolation of CD4+, CD8+, and pan T cells from different mouse tumor models using mouse CD4 (TIL) MicroBeads, CD8 (TIL) MicroBeads, and CD4/CD8 (TIL) MicroBeads dramatically decreases time of analysis.
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