Antigens for SARS-CoV-2 research

Antigens for SARS-CoV-2 research

The scientific community is relentlessly working to investigate and fight SARS-CoV-2. Miltenyi Biotec stands committed to assisting those researchers in getting on top of COVID-19. 

SARS-CoV-2 antigens and ACE2


SARS-CoV-2 antigens offer researchers the possibility to investigate virus-specific immune responses, including antigen-specific B cells and antibodies.  
 

Overview of our antigens for SARS-CoV-2 research. All antigens are available with or without biotinylation:

SARS-CoV-2 variantProtein   Sequence partProduct page
n/aACE2Full length (with CLD)Recombinant Human ACE2 (HEK)
Truncated (w/o CLD)Recombinant Human ACE2 (insect cells)
Wuhan wild-type variantSpike proteinSpike trimer (ectodomain)Recombinant SARS-CoV-2 Spike-Trimer (HEK)
Spike monomer (ectodomain)Recombinant SARS-CoV-2 Spike-Prot (HEK)
Furin cleavage site (and control)SARS-CoV-2 Furin Cleavage Site
Receptor binding domain (RBD)Recombinant SARS-CoV-2 RBD (HEK)
Recombinant SARS-CoV-2 RBD (insect cells)
S1Recombinant SARS-CoV-2 Spike-S1 (HEK)
S2 Recombinant SARS-CoV-2 Spike-S2 (insect cells)
NucleoproteinFull lengthRecombinant SARS-CoV-2 Nucleoprotein
Envelope proteinC-terminusSARS-CoV-2 Envelope C-term
N-terminus SARS-CoV-2 Envelope N-term
Helical segment 2 (H2)SARS-CoV-2 Envelope H2
B.1.1.7 variant Spike proteinRBDRecombinant SARS-CoV-2 RBD B.1.1.7 (HEK)
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Find relevant products for analysis, enrichment, and culture of SARS-CoV-2–specific B cells

Are you interested in SARS-CoV-2–specific T cell research? Check out our SARS-CoV-2 PepTivator® Peptide Pools.

Applications of SARS-CoV-2 antigens

Overview of possible applications

  • Detection of patient-derived antibodies
  • Characterization of patient-specific immune responses
  • Detection and phenotyping of virus-specific B cells
  • Enrichment of virus-specific B cells
  • Analysis of virus binding to host cells
  • Protein detection assays

Detection of SARS-CoV-2–specific antibodies in serum samples

Detection of patient-derived antibodies against SARS-CoV-2 antigens via a bead-based immunoassay 

Biotinylated recombinant proteins coupled to streptavidin-coated PMMA beads can be applied to detect SARS-CoV-2–specific antibodies in plasmapheresis samples of COVID-19+ patients. The complete protocol can be found here.

Analysis of patient-derived SARS-CoV-2 antibodies
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Analysis of different plasma dilutions illustrates that our SARS-CoV-2 antigens enable a highly sensitive detection of patient-derived anti–SARS-CoV-2 antibodies. 

Quantification of captured SARS-CoV-2 antibodies at different plasma dilutions
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Quantification of captured SARS-CoV-2 antibodies at different plasma dilutions shows the high specificity of our SARS-CoV-2 antigens.

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Knowing antibody titers is often the first step before analyzing the cellular immune response to an infection. To get an overview about our workflows for detection of humoral and cellular responses to SARS-CoV-2 download our scientific poster.

Detection of spike protein binding to ACE2

Cell-based assay to analyze the binding of spike protein to native ACE2

The combination of recombinant spike RBD, monomer or trimer, and ACE2-expressing cells can be used to mimic the binding of SARS-CoV-2 to its host cells in vitro. Such binding assays can be applied to study inhibitory/neutralizing effects of components or antibodies.

Analysis of spike protein binding to cell surface ACE2 
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Immunofluorescent staining visualizes spike proteins (RBD, monomer, trimer) bound to ACE2-expressing cells. Comparison of the percentage of stained cells at different protein concentrations shows a titratable, high-affinity binding of all three recombinant spike proteins to cell surface ACE2, with the trimer showing the highest affinity.  

This assay can be applied to analyze the potential of certain components to disrupt spike/ACE2 binding, which would result in a reduced number of stained cells. 

Furin cleavage screening assay 

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Application note
Furin cleavage screening assay 

Furin cleavage of the S protein is considered a promising therapeutic target. Download this application note to see how our furin cleavage site peptides can be applied in a fluorogenic assay to screen for furin cleavage.

Available antigens for your SARS-CoV-2 research

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If you have further questions or are interested in our proteins for SARS-CoV-2 research, please specify your question or request in the contact form and we will come back to you:

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