Peptide pools for SARS-CoV-2–specific T cell research

Peptide pools for SARS-CoV-2–specific T cell research

The scientific community is relentlessly working to fight SARS‑CoV‑2. Miltenyi Biotec stands committed to assisting those researchers in getting on top of COVID-19. 

SARS‑CoV‑2 PepTivator® Peptide Pools

SARS-CoV-2 PepTivator Peptide Pools can be used to load antigen-presenting cells and to stimulate SARS-CoV-2–specific T cells. The T cells can then be detected and/or isolated for further research, including analysis of T cell immunity after vaccination or monitoring the immune status after infection. We provide different peptide pools, covering the sequence of major structural SARS-CoV-2 proteins. 
 

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SARS‑CoV‑2 PepTivator Peptide Pools – a short introduction

Get to know our products in this video! 

See how the SARS‑CoV‑2 PepTivator Peptide Pools work and how they can potentially be applied in COVID-19 research.

New: PepTivator SARS-CoV-2 Select, covering immunodominant epitopes of structural as well as non-structural proteins, available in premium grade and MACS® GMP Quality. Get more product information here.

Exemplary application data of SARS‑CoV‑2 PepTivator Peptide Pools

Detection of SARS-CoV-2–specific CD3+ T cells in recovered COVID-19 patients

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Recovered COVID-19 patients show elevated levels of IFN-γ–producing CD3+ T cells upon stimulation with SARS-CoV-2 PepTivator Peptide Pools 

Stimulation with SARS-CoV-2 PepTivator Peptide Pools shows the presence of virus-specific T cells in recovered COVID-19 patients. After stimulation with the peptide pools, intracellular IFN-γ was stained and analyzed in CD3+ T cells using flow cytometry. 

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Exemplary quantitative analysis of SARS-CoV-2–specific CD3+ T cells 

Quantitative analysis of SARS‑CoV‑2–specific T cells highlights that the highest numbers of activated T cells can be observed when SARS-CoV-2 PepTivator Prot_N, Prot_M, and Prot_S are combined.

Detection of SARS-CoV-2–specific CD4+ and CD8+ T cells in recovered COVID-19 patients  

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SARS‑CoV‑2 PepTivator Peptide Pools efficiently stimulate CD4+ and CD8+ T cells 

Stimulation with a mix of SARS-CoV-2 PepTivator Peptide Pools shows the presence of virus-specific CD4+ and CD8+ T cells in recovered COVID-19 patients. Following the stimulation with the mixed peptide pools, CD154 and TNF-α expression was analyzed for CD4+ T cells, whereas expression of TNF-α and IFN-γ was analyzed for CD8+ T cells. Analysis was done using flow cytometry according to the protocol of our new SARS-CoV-2 T Cell Analysis Kit (PBMC), human.

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Our new PepTivator SARS‑CoV‑2 Select induces a strong CD4+ and CD8+ T cell response  

Our new PepTivator SARS-CoV-2 Select covers immunodominant epitopes from structural proteins (spike protein, membrane protein, nucleoprotein, and envelope protein) as well as non-structural proteins of the virus. 
Stimulation with the PepTivator SARS-CoV-2 Select leads to the stimulation of virus-specific CD4+ and CD8+ T cells in recovered COVID-19 patients as shown by flow cytometry analysis. 
 

In these exemplary data, the mix of SARS-CoV-2 PepTivator Peptide Pools (Prot_N, Prot_M, and Prot_S) was used to stimulate cells in PBMC samples, whereas the PepTivator SARS-CoV-2 Select was used to stimulate T cells in whole blood samples. The results illustrate that SARS-CoV-2–reactive T cells can be detected in PBMC samples as well as directly in whole blood upon stimulation with PepTivator Peptide Pools.  
 

Enrichment of SARS-CoV-2–specific T cells

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Exemplary enrichment of SARS‑CoV‑2–specific T cells  

Rare SARS‑CoV‑2–specific T cells can be enriched using Rapid antigen-specific T cell enrichment (Rapid ARTE). Samples were stimulated with mixed SARS‑CoV‑2 PepTivator Peptide Pools, stained (CD3, CD4, CD8, CD154, and TNF-α), and enriched based on the Rapid ARTE protocol.  
 

Data are kindly provided by Alexander Scheffold and Petra Bacher, UKSH, Kiel, Germany. 

Please note that data are derived from a single experiment and do not include multiple donors. Thus, the results should be considered as exemplary only and not as representative. Results may vary dependent on donor, time since infection, and used assays. 

Related documents:

Selected references:

  • Thieme, C. J. et al. (2020) The SARS-CoV-2 T cell immunity is directed against the spike, membrane, and nucleocapsid protein and associated with COVID-19 severity. medRxiv 2020.05.13.20100636. Unrefereed preprint.
  • Ziwei, L. (2020) SARS-CoV-2–specific T cell memory is long-lasting in the majority of convalsecent COVID-19 individuals. bioRxiv 2020.11.15.383463. Unrefereed preprint.
  • Haun, B. K. et al. (2020) CoVaccine HT™ adjuvant potentiates robust immune responses to recombinant SARS-CoV-2 Spike S1 immunisation. bioRxiv 2020.07.24.220715. Unrefereed preprint.

More references using our SARS-CoV-2 PepTivator Peptide Pools can be found in the reference section below.

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