With MACSxpress® Technology, direct isolation of immune cells from human blood has never been easier. It allows processing of large blood volumes of up to 30 mL in one single tube. Without the need for density gradient centrifugation, this technology offers a fast and easy way to isolate viable and functional immune cells from various blood products.
Using one of our MACSxpress Isolation Kits, you get highly pure target cells within 25 minutes. In this example, neutrophils were isolated from whole blood using the MACSxpress Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator.
Untouched neutrophils were isolated from 8 mL of human EDTA anticoagulated whole blood using the MACSxpress® Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD14-PerCP, CD15-PE, CD16-APC, CD45-VioBlue®, and CD193-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.