Vio® Bright V423 fluorophore

Vio Bright V423 for violet lasers

Vio Bright V423 is Miltenyi Biotec’s brightest dye excited at 405 nm. In addition to high brightness this dye shows a significantly improved signal-to-noise ratio, minimal intralaser compensation and a superior detection of rare antigens.  
Vio Bright V423 is a synthetic dye which is 5-times brighter compared to the VioBlue® Dye. This makes Vio Bright V423 an excellent choice for multiparameter cell analysis. 

 

Fluorophore at one glance

LaserViolet (405 nm)
Excitation max399 nm
Emission max420 nm
ApplicationFlow cytometry
Common filter set (FC)450/50 nm
Stain index 5 out of 5
PhotostabilityPhotostable to ambient light
Fixation stability PFA

Vio Bright V423 is a superior alternative to:

  • Brilliant Violet™ 421
  • Super Bright 436
 

Characteristics of Vio Bright V423

Absorption and emission spectra of Vio Bright V423

  • Compatible with all instruments equipped with a violet (405 nm) laser and a standard 450/50 nm filter set
  • Similar emission spectra to Brilliant Violet 421 and Super Bright 436

Brighter and clearer staining for violet laser

  • Enhanced or similar brightness compared to Brilliant Violet 421 and Super Bright 421
  • Enhanced brightness of Vio Bright V423 conjugates across different antigens

High specificity of fluorescent signal

  • Exceptional brightness allows for staining of rare cells and dim markers, e.g. CD25
  • Even better brightness than Vio Bright B515

Decreased spillover

  • Minimal spillover into neighbouring detection channels
  • Minimal change in compensation settings required

Compensation matrix for a CD19 antibody conjugated to different fluorochromes excited by a violet laser. Values depict the percentage of compensation required for the respective detection filter.

 ConjugateV1-A 
(450/50nm)
V2-A 
(525/50 nm) 
V3-A 
(579/34 nm)
V4-A 
(615/20 nm)
V5-A 
(667/30 nm) 
V1-A (450/50 nm) CD19-Vio Bright V423 10041  
V2-A (525/50 nm) CD19-BV510 4010060406
V3-A (579/34 nm) CD19-BV5704031006510
V5-A (667/30 nm) CD19-BV65030 352100

Excellent fixation stability

  • Excellent stability after fixation with paraformaldehyde
  • Compatible with different surface and intracellular staining protocols and buffer sets
  • Conjugate performance may vary depending on antigen nature upon fixation

High photostability

  • High photostability
  • Only minor drop in mean fluorescent intensity (MFI) and stain index (SI) after 24 h at continuous exposure to ambient light (~850 lux)
 

Resources

 Fluorescence spectra viewer 

Check out our fluorescence spectra viewer for well-planned multicolor flow cytometry experiments.

 

All Vio Bright V423 Products

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