The Treg Expansion Kit was developed for the efficient expansion of human Treg cells.

Data and images for Treg Expansion Kit, human

Figures

Figure 1

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Expansion rates of Treg cells were calculated from the numbers of FoxP3-expressing cells before and after 14 days of expansion in the absence or presence of rapamycin.

Figure 1

Expansion rates of Treg cells were calculated from the numbers of FoxP3-expressing cells before and after 14 days of expansion in the absence or presence of rapamycin.

Specifications for Treg Expansion Kit, human

Overview

The Treg Expansion Kit was developed for the efficient expansion of human Treg cells.

Detailed product information

Background information

Using this kit FoxP3 expression by Treg cells is sustained after isolation with the CD4
+
CD25
+
Regulatory T cell Isolation Kit, CD4
+
CD25
+
CD127
dim/–
or CD4
+
CD25
+
CD45RA
+
Regulatory T Cell Isolation Kits from human blood or PBMCs.
1
The kit is also suitable for usage with rapamycin.
2
The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 4:1 and recombinant interleukin 2 (rIL-2) with a concentration of 500 U/mL.

Downstream applications

Expansion of human Treg cells after isolation with the CD4
+
CD25
+
CD127
dim/–
or CD4
+
CD25
+
CD45RA
+
Regulatory T Cell Isolation Kits from human blood or PBMCs. Expanded Treg cells can be used for any downstream application such as cytokine analysis, gene expression, and suppression assays.

References for Treg Expansion Kit, human

Publications

  1. Hoffmann, P. et al. (2006)
    Only the CD45RA
    +
    subpopulation of CD4
    +
    CD25
    high
    T cells gives rise to homogeneous regulatory T-cell lines upon
    in vitro
    expansion.
    Blood 108: 4260-4267
  2. Battaglia, M. et al. (2005)
    Rapamycin selectively expands CD4
    +
    CD25
    +
    FoxP3
    +
    regulatory T cells.
    Blood 105: 4743-4748

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