Name: StraightFrom® Leukopak® CD8 MicroBead Kit, human
Availability: InStock

Product data and images for StraightFrom
^®
Leukopak
^®
CD8 MicroBead Kit
, human

Figures

Figure 1

Separation of a Leukopak

^®

sample using the StraightFrom

^®

Leukopak

^®

CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

CD8

⁺

T cells

Figure 1

Separation of a Leukopak

^®

sample using the StraightFrom

^®

Leukopak

^®

CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a Leukopak

^®

sample using the StraightFrom

^®

Leukopak

^®

CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for StraightFrom
^®
Leukopak
^®
CD8 MicroBead Kit
, human

Overview

The StraightFrom

^®

Leukopak

^®

MicroBead Kit was developed for the rapid positive selection of CD8

⁺

cells directly from Leukopak

^®

using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Block and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak

^®

Detailed product information

Background information

In blood, the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16

⁺

NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8

⁺

cytotoxic T cells.

Detailed separation procedure

A Leukopak

^®

sample is labeled with StraightFrom

^®

Leukopak

^®

CD8 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

^®

Separator. After removing the column from the magnetic field, the magnetically retained CD4

⁺

T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8

⁺

cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block

Resources for StraightFrom
^®
Leukopak
^®
CD8 MicroBead Kit
, human

Documents and Protocols

Brochures/posters

StraightFrom® MicroBeads - Cell isolation directly from blood products

Scientific posters

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®

App notes/customer reports

Fast and efficient T cell isolation without density gradient centrifugation for CAR T cell engineering

Data and images

Data and images for StraightFrom
^®
Leukopak
^®
CD8 MicroBead Kit
, human

Figures

Figure 1

Separation of a Leukopak

^®

sample using the StraightFrom

^®

Leukopak

^®

CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

CD8

⁺

T cells

Figure 1

Separation of a Leukopak

^®

sample using the StraightFrom

^®

Leukopak

^®

CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a Leukopak

^®

sample using the StraightFrom

^®

Leukopak

^®

CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for StraightFrom
^®
Leukopak
^®
CD8 MicroBead Kit
, human

Overview

The StraightFrom

^®

Leukopak

^®

MicroBead Kit was developed for the rapid positive selection of CD8

⁺

cells directly from Leukopak

^®

Detailed product information

Background information

In blood, the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16

⁺

NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8

⁺

cytotoxic T cells.

Detailed separation procedure

A Leukopak

^®

sample is labeled with StraightFrom

^®

Leukopak

^®

CD8 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

^®

Separator. After removing the column from the magnetic field, the magnetically retained CD4

⁺

T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8

⁺

cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block

Resources

Resources for StraightFrom
^®
Leukopak
^®
CD8 MicroBead Kit
, human

Documents and Protocols

Brochures/posters

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

StraightFrom® Leukopak® CD8 MicroBead Kit, human

StraightFrom® Leukopak® CD8 MicroBead Kit, human

Product data and images for StraightFrom® Leukopak® CD8 MicroBead Kit, human

Figures

Figure 1