Ready-to-use multiplex kits for flow cytometry

  • High-parameter measurements with up to 39 specificities at the same time
  • Perfect match with MACSQuant® Analyzers and Express Modes for fully automated experiment setup, acquisition, and analysis
  • Time-saving method compared to other flow cytometry-based multiplex assays

Available options

Overview of available MACSPlex Kits

Application categoryKit nameNumber of analytesTarget analytesMACSQuantify Software versionExpress Mode package
Human cytokinesMACSPlex Cytokine 12 Kit, human12IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α2.5 and higher25.2.9990 and higher
Mouse cytokinesMACSPlex Cytokine 10 Kit, mouse10IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α2.5 and higher25.3.10934 and higher
Human cytotoxicityMACSPlex Cytotoxic T/NK Cell Kit, human12IL-2, IL-4, IL-6, IL-10, IL17A, IL-21, GM-CSF, Granzyme B, IFN-γ, MCP-1, TNF-α, Perforin2.13.1 and higher213.2.20284 and higher* 
Human exosomesMACSPlex Exosome Kit, human37 exosome markers and 2 controlsCD3, CD4, CD19, CD8, HLA-DRDPDQ, CD56, CD105, CD2, CD1c, CD25, CD49e, ROR1, CD209, CD9, SSEA-4, HLA-ABC, CD63, CD40, CD62P, CD11c, CD81, MCSP, CD146, CD41b, CD42a, CD24, CD86, CD44, CD326, CD133/1, CD29, CD69, CD142, CD45, CD31, CD20, CD14REA Control, mIgG1 control2.8 and higher28.1.15443 and higher 
Human miRNAMACSPlex miRNA Kit - Cancer, human35 miRNAs and 4 controlsmiR-378b, miR-141-3p, miR-23a-3p, miR-17-5p, miR-20a-5p, miR-200a-3p, miR-29a-3p, miR-429, miR-143-3p, miR-21-5p, miR-19b-3p, miR-31-5p, let-7f-5p, miR-335-5p, miR-200b-3p, miR-34a-5p, let-7a-5p, miR-101-3p, miR-373-3p, miR-122-5p, miR-107, hsa.miR-30b-5p, miR-15b-5p, miR-520c-3p, miR-200c-3p, miR-9-5p, miR-23b-3p, let-7g-5p, miR-145-5p, let-7b-5p, miR-103a-3p, miR-10b-5p, miR-10b-5p, miR-16-5p, miR-126-3p, miR-15a-5pControl-4-Neg, Control-1-U6, Control-2-5.8S, Control-3-C42.6 and higher26.1.12747 and higher
*Note: to get the new Express Mode package please contact your local support team 

Basic principle

Principle of MACSPlex T/NK Cell Assays
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Samples containing unknown levels of analytes are incubated with the antibody-coated MACSPlex Capture Beads, and analytes bind to the specific antibody. Two detection reagents, composed of a cocktail of either biotin-conjugated or APC-conjugated antibodies specific for the analytes, are added. Consequently, sandwich complexes are formed between the MACSPlex Capture Beads, the analyte, and the Detection Reagent Biotin while the Detection Reagent APC binds to the biotinylated detection antibodies. These complexes can be analyzed based on the fluorescence characteristics. 

Standards of known quantities of given analytes are provided with the kit and are used for the quantification of the analytes within the unknown samples. 
 

Prerequisites

For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary. 
 

Analysis with MACSQuant Analyzers and Express Modes

Exemplary analysis and results using a MACSPlex Cytokine Kit
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When using the MACSQuant Analyzer 10 or MACSQuant X, our MACSplex Kits can be combined with fully automated Express Modes. 

By this, an automated gating strategy to populations of interest will be applied and the gates will be automatically adjusted for each data file individually to achieve optimal results. 

Furthermore, a one-click batch analysis of all samples belonging to one experiment can be performed to directly compare your data. The concentrations of each analyte will be presented as an exportale table as well as a ready-to-publish graph.

ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above. 

Analysis with other instruments

For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays. 

30-day trial versions are available for both Flowlogic Software and the Beadlogic Add-on

Additionally, third-party software can be used for analysis.

Resources

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Application note

General instructions for MACSPlex Cytokine Kits

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Application note

General instructions for MACSPlex miRNA Kit – Cancer

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Application note

MACSPlex Filter Plate calibration

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Protocol

Fixation of exosomes on MACSPlex Exosome Capture Beads

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