The monoclonal His antibody facilitates the fast and convenient detection of His-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. Fluorochrome-conjugated His antibodies enable direct flow cytometry and fluorescent microscopy analyses, while indirect fluorescent labeling of His fusion protein expressing cells is possible with the biotin-conjugated antibody. The monoclonal His antibody specifically recognizes proteins that contain a polyhistidine tag at their N- and C-terminus. However, His Antibody, HRP (C-term.) shows higher sensitivity and specificity for C-terminal His-tagged proteins.
