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Miltenyi Biotec-tested panel 32 (MBTP32)
This application protocol describes the analysis of differentiation stages of human γδ T cells in peripheral blood mononuclear cells (PBMCs) using flow cytometry.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD3||REA613||T cells||Vio® Bright R720||725/40 (red)|
|CD4||REA623||T helper cells||PE-Vio 770||785/62 (blue)|
|CD8||REA734||Cytotoxic T cells||APC-Vio 770||785/62 (red)|
|CD28||REA612||Differentiating γδ T cells||PE||579/34 (blue)|
|CD27||REA499||Differentiating γδ T cells||APC||667/30 (red)|
|TCRγ/δ||REA591||γδ T cells||FITC||525/50 (blue)|
|TCR Vδ1||REA173||γδ T cell subpopulation||VioGreen™||525/50 (violet)|
|TCR Vδ2||REA771||γδ T cell subpopulation||PerCP-Vio 700||725/40 (blue)|
|CD45RA||REA562||Differentiating γδ T cells||Vio Bright V423||450/50 (violet)|
|CD16||REA423||γδ T cell activation||PE-Vio 615||615/20 (blue)|
|7-AAD Staining Solution||-||Viability||-||667/30 (blue)|
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