Comprehensive solutions for your Treg human and mouse research

We provide the means for a fast and gentle isolation and flow sorting of regulatory T cells (Treg cells) as well as distinct Treg subsets. You have the possibility of optimized culture, activation, and expansion, as well as phenotyping and functional analysis of Treg cells.

Research Treg workflow

Our workflows are synergistic and fully translational to clinical Treg applications.

Webinar
Comprehensive solutions for your Treg mouse and human research

Regulatory T cells (Tregs) are an important T cell subpopulation that maintains immunological homeostasis. Today, Treg-based therapy shows an enormous potential for treatment of autoimmune and auto-inflammatory disorders, acute and chronic infection, allergy, metabolic inflammation, transplantation, and cancer. Therefore, it is crucial to provide reliable and trustworthy research materials alongside an easily translatable workflow into the clinic. This webinar explores our innovative tools to support Treg research at every step.

Analysis of CD4+FoxP3+Treg cells in cell suspensions of lamina propria from mouse colons by flow cytometry using Flowlogic™ software.

Tregs from mouse tissue

Tregs represent 1–4% of all lymphocytes in secondary lymphoid organs (such as spleen and lymph node) of wild type mice. Spleen and lymph nodes must be dissociated into a single-cell suspension for many downstream applications. Dissociation can be accomplished with specific Tissue Dissociation Kits (e.g. Spleen Dissociation Kit, mouse) manually or in a fast and convenient way by using the automatic gentleMACS™ Dissociator.

CD4+CD25+CD127dim/– regulatory T cells isolated from human PBMCs.

Cell isolation

We offer a broad range of products for the isolation of Treg cells according to your starting material and species; human, mouse, and non-human primate. MACS® MicroBeads and Isolation Kits are the best products for isolation of highly pure and viable distinct Treg subsets.

 

MACS® MicroBead Technology is the most trusted and proven cell isolation technology. 

Isolation of human CD4+CD25+CD127dim/-Regulatory T Cell using MACS® Technology

  • Minimal labeling and smallest bead size – preserved characteristics and no cell activation.
  • No cell stress – highest viability and no effects on cell functionality.
  • Free epitopes and no bead aggregates – cells can be directly used in downstream applications.

 

Isolation of CD4+CD25+ Treg cells using the MACSxpress® Treg Isolation Kit

Our MACSxpress® Treg Isolation Kit, human has been developed for the isolation of CD4+CD25+ Treg cells from up to 30 mL of freshly drawn anti-coagulated whole blood. Isolate pure Treg cells in only 30 minutes, without the requirement of density gradient centrifugation. 

 

Isolation of mouse Treg cells using the CD4+CD25+ Regulatory T Cell Isolation Kit, mouse.

Isolation of mouse Treg cells using the CD4+CD25+ Regulatory T Cell Isolation Kit, mouse

Human Treg sorting by MACSQuant® Tyto® CellSorter. 

Sorting of Tregs with MACSQuant® Tyto® Cell Sorter

Tregs are fragile and sensitive to mechanical stress. Therefore, gentle isolation is a critical step for accurate downstream (functional and phenotypical) analyses and applications.

The MACSQuant® Tyto® Cell Sorter is revolutionizing cell sorting. Our patented microchip-based technology opens up new possibilities in basic research and medical applications with high-speed multiparameter cell sorting in the safety of a fully enclosed and sterile cartridge system, the MACSQuant Tyto Cartridge.

The MACSQuant Tyto Cell Sorter gives you the opportunity to sort different subtype of Tregs not only for your research purposes but also for cell and gene therapy application. 

Human Treg sorting by MACSQuant® Tyto® CellSorter:

  • Gentle to cells: Sort and even resort cells under low pressure without compromising cell viability or functionality.
  • Fast and easy handling: No drop delay or laser alignment needed.
  • Full sterility: Contamination-free within the disposable, fully closed MACSQuant Tyto Cartridge.

REAfinity™ Recombinant Antibodies provide complete multiparameter labeling for your Tregs research Flow cytometry and cell sorting analysis. However, REAlease® Fluorochrome Technology not only supports you with complete multiparameter labeling, but provides unlabeled Tregs after the cell sorting.

Expansion of Treg cells using the Treg Expansion Kit, human. 

Activation, expansion, and stimulation of Treg cells can be very challenging. The combination of TexMACS™ Medium, MACS® Cytokines, and Treg expansion kits (human and mouse) are perfectly suited for in vitro culture and expansion of Treg cells.

TexMACS™ Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells.

Optimal expansion of your isolated Treg cells using Treg Expansion Kit 

  • Based on cell-sized MACSiBead™ Particles loaded with CD3 and CD28 antibodies
  • Optimized to perfection and a careful balance of the stimuli enables reliable Tregs expansion
  • Maintenance of Tregs phenotype and FoxP3 expression in vitro.
Treg suppression assay using the Treg Suppression Inspector, human

Treg suppression assay

The Treg Suppression Inspector has been developed for the in vitro functional characterization of human CD4+CD25+regulatory T cells.

Treg in vitro suppression assay 

Treg suppression assay using the Treg Suppression Inspector, human:

  • An optimized T cell stimulation reagent for a Treg suppression assay
  • Based on Anti-Biotin MACSiBead™ Particles
  • Analysis of Tregs suppression activity by reduction of responder T cells proliferation
REAfinity™ Recombinant Antibody model.

Flow cytometry analysis 

Treg Detection Kits (mouse and human) 

The Treg Detection Kits (mouse and human) are optimized antibody kits with pre-titrated antibodies that ensure a reliable flow cytometric analysis of Treg cells. Kits are ready-to-use cocktail, which conveniently reduces pipetting steps.

 

REAfinity™ Recombinant Antibodies

REAfinity™ Antibodies are recombinant antibodies that provide superior lot-to-lot consistency and purity compared to mouse or rat hybridoma-derived, monoclonal antibodies.

REAfinity™ Recombinant Antibody model:

  • Greater reproducibility: High purity and lot-to-lot consistency with less background
  • Easy handling: Eliminates tedious and costly Fc receptor blocking steps
  • One universal isotype: Convenient and cost-saving

Scientific poster
Vio® Dyes meet REAfinity™ Antibodies – antibody-fluorochrome conjugates for high-resolution multiparameter flow cytometry analysis

Cesar Evaristo, Marco Vahldieck, Susanne Krauthaeuser, Nicole Jansen, Niklas Wilske, Anne Richter, and Christian Dose

Get acquainted with our protocols and antibody panels for your whole workflow in  your Treg research. 

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