The Treg Suppression Inspector has been developed for the functional characterization of human CD4
+
CD25
+
regulatory T (Treg) cells by
in vitro
suppression assays.

Data and images for Treg Suppression Inspector, human

Figures

Figure 1

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CD4
+
CD25
+
regulatory T cells, isolated with the CD4
+
CD25
+
Regulatory T Cell Isolation Kit, were cocultured with CD4
+
CD25
-
responder T cells in different ratios. For T cell stimulation, the Treg Suppression Inspector was added to the culture. As controls, CD4
+
CD25
+
Treg cells and CD4
+
CD25
-
responder T cells alone were cultured without any stimulus. Proliferation of T cells was determined by measuring
3
H-thymidine incorporation.

Figure 1

CD4
+
CD25
+
regulatory T cells, isolated with the CD4
+
CD25
+
Regulatory T Cell Isolation Kit, were cocultured with CD4
+
CD25
-
responder T cells in different ratios. For T cell stimulation, the Treg Suppression Inspector was added to the culture. As controls, CD4
+
CD25
+
Treg cells and CD4
+
CD25
-
responder T cells alone were cultured without any stimulus. Proliferation of T cells was determined by measuring
3
H-thymidine incorporation.

Specifications for Treg Suppression Inspector, human

Overview

The Treg Suppression Inspector has been developed for the functional characterization of human CD4
+
CD25
+
regulatory T (Treg) cells by
in vitro
suppression assays.

Detailed product information

Background information

Treg cells are a subset of T cells that have the ability to suppress harmful immunological reactions to self or foreign antigens. This function of Treg cells can be analyzed by a suppression assay. For this purpose, Treg cells and responder T cells are cocultured in different ratios in the presence of an optimized polyclonal stimulus, the Treg Suppression Inspector, while the proliferative response is measured.
The Treg Suppression Inspector is an optimized T cell stimulation reagent for a Treg suppression assay, which is based on Anti-Biotin MACSiBead™ Particles that are loaded with biotinylated CD2, CD3, and CD28 antibodies.
Activation of responder T cells (CD4
+
CD25
-
or CD4
+
T cells) with MACSiBead™ Particles leads to proliferation, whereas Treg cells in the presence of the stimulus are not activated. Coculture of responder T cells, MACSiBead™ Particles and Treg cells results in reduced proliferation of responder T cells due to suppression by Treg cells.

Resources for Treg Suppression Inspector, human

References for Treg Suppression Inspector, human

Publications

  1. Kleinewietfeld et al. (2009) CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells. Blood 113: 827-836
  2. Mougiakakos, D. et al. (2009) Naturally occurring regulatory T cells show reduced sensitivity toward oxidative stress-induced cell death. Blood 113: 3542-3545

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