Reliable analysis of rare virus-specific T cells
T cells play a pivotal role in fighting viral infections and generating long-term protective immunity after infection and vaccination.
Assessment of specificity, phenotype, and cytokine secretion of rare virus-reactive T cells can provide essential information prior infections, on infection progression, or on vaccination efficacy.
Isolation of PBMCs or T cells from human whole blood
Choose between our solutions for manual or automated cell isolation from human whole blood.
- The MACSprep PBMC Isolation Kit, human enables a safe and fast untouched isolation of human PBMCs from small whole blood samples without density-gradient centrifugation.
- StraightFrom® MicroBeads allow for direct isolation of T cells from whole blood and blood products.
- Automated cell isolation solutions for large sample numbers.
Virus-specific in vitro stimulation of T cells and other immune cells
We offer PepTivator® Peptide Pools for efficient antigen-specific stimulation of both CD4+ and CD8+ T cells, as well as recombinant SARS-CoV-2 proteins for stimulation of other immune cells.
- SARS-CoV-2 PepTivator Peptide Pools for SARS-CoV-2–specific stimulation of T cells.
- Recombinant SARS-CoV-2 proteins are highly immunogenic and can be used for the stimulation of various immune cells.
Application note
Fast and efficient detection of SARS-CoV-2–reactive T cells via antigen-specific stimulation
See how we used our new SARS-CoV-2 T Cell Analysis Kit (PBMC), human for the stimulation and analysis of virus-reactive T cells in the context of COVID-19.
Magnetic enrichment of antigen- and virus-specific T cells
Antigen- and virus-specific T cells are extremely rare, which makes analysis difficult. Our optimized enrichment solutions enable you to analyze even the rarest cells.
- CD137 MicroBead Kit, human and CD154 MicroBead Kit, human for the pre-enrichment of rare virus-reactive T cells based on activation marker expression.
- IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human to detect and enrich viable IFN-γ–secreting T cells for any downstream application. Other specificities are available.
Protocol
Rapid antigen-specific T cell enrichment (Rapid ARTE)
Combine magnetic cell enrichment and multiparameter flow cytometric analysis for direct ex vivo characterization of human antigen-specific CD154+CD4+ T cells.
Safe flow sorting of antigen- and virus-specific T cells
When working with contaminated or infectious material, operator safety is of utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of rare virus-specific T cells in a closed aerosol-free cartridge system.
- The MACSQuant Tyto Cell Sorter for safe, gentle and sterile sorting of infectious material.
- Discover exciting customer stories of overcoming challenges in cell sorting.
Scientific poster
Detection of humoral and cellular responses to SARS-CoV-2 in COVID-19 convalescents
Download our scientific poster to see how the MACSQuant Tyto can be used for sorting of SARS-CoV-2–specific B cells with fluorescent SARS-COV-2 antigen-tetramers.
Multiparameter flow analysis of antigen- and virus-specific T cells
Our MACS® Flow Cytometry Kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility, and most precise results. The MACSQuant Analyzers with automated MACSExpress® Analysis Modes eliminate operator variability and standardize your analysis further.
- SARS-CoV-2 T Cell Analysis Kits are true all-in-one kits for the efficient stimulation and analysis of human SARS-CoV-2–reactive T cells. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator Peptide Pool of choice and an optimized flow panel (see table below).
- REAfinity™ Recombinant Antibodies are recombinantly engineered for higher purity, superior lot-to-lot consistency, and lowest background.
- MACSplex Cytokine Kits for a multiplexed analysis of secreted cytokines in cell culture supernatant.
- Rapid Cytokine Inspector Kits are unique kits that offer the fastest combination of surface marker and intracellular cytokine staining.
- 8-Color Immunophenotyping Kit, anti-human, REAfinity for reliable identification of leukocyte subsets in one simple kit, e.g., monocytes, neutrophils, eosinophils, and T, B, and NK cell populations.
- StainExpress™ Immune Cell Composition Cocktail, human to achieve highest standardization. These dried antibody cocktails enable reliable evaluation of immune cell composition from human blood material (e.g. whole blood or PBMCs).
- MACSQuant® Analyzers are fully automated benchtop flow cytometers for highly sensitive multiparameter flow analyses.
Video
Discover the beauty of automated flow cytometry
All you have to do is choose your cells and markers of interest: Our family of MACSQuant Analyzers for different applications can handle the rest.
Optimized flow panel for SARS-CoV-2 T Cell Analysis
| Marker | Clone | Fluorophore |
|---|---|---|
| CD3 | REA613 | APC |
| CD4 | REA623 | Vio® Bright B515 |
| CD8 | REA734 | VioGreen™ |
| CD154 | REA238 | APC-Vio® 770 |
| IFN-g | REA600 | PE |
| IL-2 | REA689 | PE-Vio® 615 |
| TNF-a | REA656 | PE-Vio® 770 |
| CD14 | REA599 | VioBlue® |
| CD20 | REA780 | VioBlue |
| Live/dead | Viobility™ Fixable Dye | 405/452 |
Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.*
For Vio Dye spectra see here
