Reliable analysis of rare virus-specific T cells 

T cells play a pivotal role in fighting viral infections and generating long-term protective immunity after infection and vaccination. 

Assessment of specificity, phenotype, and cytokine secretion of rare virus-reactive T cells can provide essential information prior infections, on infection progression,  or on vaccination efficacy. 

Product highlight

SARS-CoV-2 T Cell Analysis Kits

All-in-one SARS-CoV-2 T Cell Analysis Kits to monitor and analyze human SARS-CoV-2–reactive T cell subsets from PBMC or whole blood samples. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator® Peptide Pool of choice and an optimized flow cytometry panel.

SARS-CoV-2 T Cell Analysis Kits

A complete workflow for stimulation, enrichment, and analysis of virus-reactive T cells

Isolation of PBMCs or T cells from human whole blood

Choose between our solutions for manual or automated cell isolation from human whole blood.  

Virus-specific in vitro stimulation of T cells and other immune cells 

We offer PepTivator® Peptide Pools for efficient antigen-specific stimulation of both CD4+ and CD8+ T cells, as well as recombinant SARS-CoV-2 proteins for stimulation of other immune cells. 

Application note
Fast and efficient detection of SARS-CoV-2–reactive T cells via antigen-specific stimulation

See how we used our new SARS-CoV-2 T Cell Analysis Kit (PBMC), human for the stimulation and analysis of virus-reactive T cells in the context of COVID-19.

Magnetic enrichment of antigen- and virus-specific T cells

Antigen- and virus-specific T cells are extremely rare, which makes analysis difficult. Our optimized enrichment solutions enable you to analyze even the rarest cells.

Rapid antigen-specific T cell enrichment (Rapid ARTE)

Combine magnetic cell enrichment and multiparameter flow cytometric analysis for direct ex vivo characterization of human antigen-specific CD154+CD4+ T cells.

Safe flow sorting of antigen- and virus-specific T cells

When working with contaminated or infectious material, operator safety is of utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of rare virus-specific T cells in a closed aerosol-free cartridge system.

Scientific poster
Detection of humoral and cellular responses to SARS-CoV-2 in COVID-19 convalescents

Download our scientific poster to see how the MACSQuant Tyto can be used for sorting of SARS-CoV-2–specific B cells with fluorescent SARS-COV-2 antigen-tetramers. 

Multiparameter flow analysis of antigen- and virus-specific T cells

Our MACS® Flow Cytometry Kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility, and most precise results. The MACSQuant Analyzers with automated MACSExpress® Analysis Modes eliminate operator variability and standardize your analysis further.

Discover the beauty of automated flow cytometry 

All you have to do is choose your cells and markers of interest: Our family of MACSQuant Analyzers for different applications can handle the rest. 

Optimized flow panel for SARS-CoV-2 T Cell Analysis

CD4REA623 Vio® Bright B515
CD8REA734 VioGreen™
CD154REA238APC-Vio® 770
IL-2REA689 PE-Vio® 615
TNF-a REA656PE-Vio® 770
CD14REA599 VioBlue®
CD20REA780 VioBlue 
Live/dead Viobility™ Fixable Dye 405/452 

Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.*

For Vio Dye spectra see here

Check out more supporting materials on antigen-specific T cells

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