T cells play a pivotal role in fighting viral infections and generating long-term protective immunity after infection and vaccination.
Assessment of specificity, phenotype, and cytokine secretion of rare virus-reactive T cells can provide essential information prior infections, on infection progression, or on vaccination efficacy.
Choose between our solutions for manual or automated cell isolation from human whole blood.
We offer PepTivator® Peptide Pools for efficient antigen-specific stimulation of both CD4+ and CD8+ T cells, as well as recombinant SARS-CoV-2 proteins for stimulation of other immune cells.
See how we used our new SARS-CoV-2 T Cell Analysis Kit (PBMC), human for the stimulation and analysis of virus-reactive T cells in the context of COVID-19.
Antigen- and virus-specific T cells are extremely rare, which makes analysis difficult. Our optimized enrichment solutions enable you to analyze even the rarest cells.
Combine magnetic cell enrichment and multiparameter flow cytometric analysis for direct ex vivo characterization of human antigen-specific CD154+CD4+ T cells.
When working with contaminated or infectious material, operator safety is of utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of rare virus-specific T cells in a closed aerosol-free cartridge system.
Download our scientific poster to see how the MACSQuant Tyto can be used for sorting of SARS-CoV-2–specific B cells with fluorescent SARS-COV-2 antigen-tetramers.
Our MACS® Flow Cytometry Kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility, and most precise results. The MACSQuant Analyzers with automated MACSExpress® Analysis Modes eliminate operator variability and standardize your analysis further.
All you have to do is choose your cells and markers of interest: Our family of MACSQuant Analyzers for different applications can handle the rest.
Optimized flow panel for SARS-CoV-2 T Cell Analysis
Marker | Clone | Fluorophore |
---|---|---|
CD3 | REA613 | APC |
CD4 | REA623 | Vio® Bright B515 |
CD8 | REA734 | VioGreen™ |
CD154 | REA238 | APC-Vio® 770 |
IFN-g | REA600 | PE |
IL-2 | REA689 | PE-Vio® 615 |
TNF-a | REA656 | PE-Vio® 770 |
CD14 | REA599 | VioBlue® |
CD20 | REA780 | VioBlue |
Live/dead | Viobility™ Fixable Dye | 405/452 |
Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.*
For Vio Dye spectra see here
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