Tumor tissue storage and dissociation

Tumor tissue samples are often transported to the lab, for example, from collaboration sites, or they are stored while other samples are being processed. Optimal storage conditions keep tumor cells viable and prevent unwanted cell activation to enable reliable downstream analysis.

Gentle tumor dissociation is a prerequisite for efficient downstream cell separation, flow analysis, cell sorting, molecular analysis, and cell culture. Only viable single-cell suspensions with preserved cell surface epitopes will allow for reliable downstream results.

Using the gentleMACS™ Tissue Dissociators with the gentleMACS C Tubes and Tumor Dissociation Kits (human and mouse), mechanical and enzymatic dissociation are combined to enable gentle, automated tumor dissociation for standardized and reproducible results.