TexMACS Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells. This complete medium enables reproducible application in human and mouse cell culture. It is manufactured without animal-derived components.

Data and images for TexMACS™ Medium

Figures

Figure 1

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Efficient expansion of human T cells under serum-free conditions
: Human T cells were isolated using the Pan T cell Isolation Kit, human. Subsequently, cells were stimulated either with T Cell TransAct™, human (research grade), with activation beads (competitor brand), or left untreated and cultured for 14 days either in RPMI (+10% FBS) or serum-free TexMACS™ Medium. Both media were supplemented with 50 U/mL Human IL-2 IS, premium-grade.
Cell proliferation (fold expansion) was analyzed on the MACSQuant
®
Analyzer 10. Culturing in serum-free TexMACS Medium resulted in over 100-fold T cell expansion comparable to the expansion rates in RPMI + 10% FBS.

Figure 1

Efficient expansion of human T cells under serum-free conditions
: Human T cells were isolated using the Pan T cell Isolation Kit, human. Subsequently, cells were stimulated either with T Cell TransAct™, human (research grade), with activation beads (competitor brand), or left untreated and cultured for 14 days either in RPMI (+10% FBS) or serum-free TexMACS™ Medium. Both media were supplemented with 50 U/mL Human IL-2 IS, premium-grade.
Cell proliferation (fold expansion) was analyzed on the MACSQuant
®
Analyzer 10. Culturing in serum-free TexMACS Medium resulted in over 100-fold T cell expansion comparable to the expansion rates in RPMI + 10% FBS.

Figure 2

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Reliable expansion of human FoxP3+ regulatory T cells:
Human regulatory T cells (Tregs) were isolated directly from whole blood using the MACSxpress
®
Whole Blood Treg Isolation Kit, human and expanded for 21 days after activation using the Treg Expansion Kit, human. Cells were cultured in TexMACS™ Medium (suppl. with 5% AB serum, 500 U/mL IL-2 IS, Premium-Grade and 100 nmol/L MACS
®
GMP Rapamycin).
Treg cells expanded up to 40-fold in 21 days while maintaining their FoxP3
+
phenotype.

Figure 2

Reliable expansion of human FoxP3+ regulatory T cells:
Human regulatory T cells (Tregs) were isolated directly from whole blood using the MACSxpress
®
Whole Blood Treg Isolation Kit, human and expanded for 21 days after activation using the Treg Expansion Kit, human. Cells were cultured in TexMACS™ Medium (suppl. with 5% AB serum, 500 U/mL IL-2 IS, Premium-Grade and 100 nmol/L MACS
®
GMP Rapamycin).
Treg cells expanded up to 40-fold in 21 days while maintaining their FoxP3
+
phenotype.

Figure 3

View details
Expansion of mouse CD4+ T cells with and without addition of bovine serum:
Mouse CD4
+
T cells were isolated on the autoMACS
®
Pro Separator using the CD4
+
T Cell Isolation Kit, mouse, activated via the T Cell Activation/Expansion Kit, mouse, and expanded for 7 days in TexMACS™ Medium with and without FBS. Culture media were supplemented with 50 U/mL Mouse IL-2 IS, premium-grade.
Cell proliferation (fold expansion) was analyzed on the MACSQuant
®
Analyzer 10. Culturing in serum-free as well as serum-containing TexMACS™ Medium resulted 10–20-fold T cell expansion.

Figure 3

Expansion of mouse CD4+ T cells with and without addition of bovine serum:
Mouse CD4
+
T cells were isolated on the autoMACS
®
Pro Separator using the CD4
+
T Cell Isolation Kit, mouse, activated via the T Cell Activation/Expansion Kit, mouse, and expanded for 7 days in TexMACS™ Medium with and without FBS. Culture media were supplemented with 50 U/mL Mouse IL-2 IS, premium-grade.
Cell proliferation (fold expansion) was analyzed on the MACSQuant
®
Analyzer 10. Culturing in serum-free as well as serum-containing TexMACS™ Medium resulted 10–20-fold T cell expansion.

Specifications for TexMACS™ Medium

Overview

TexMACS Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells. This complete medium enables reproducible application in human and mouse cell culture. It is manufactured without animal-derived components.

Detailed product information

Background information

TexMACS Medium has been designed for high-performance T cell growth, high cell viability, and consistency under serum-free conditions. It has been optimized for the serum-free cultivation and expansion of human and mouse T cells and regulatory T cells. TexMACS Medium has a defined formulation enabling reproducible application in human and mouse cell culture. It is produced without animal-derived components, but contains pre-selected human serum albumin, stable glutamine, and phenol red. TexMACS Medium has been developed based on the TexMACS GMP Medium (# 170-076-306).

References for TexMACS™ Medium

Publications

  1. Bacher, P. et al. (2013) Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Tʜ cell repertoire. J. Immunol. 190(8): 3967-3976
  2. Saito, S. et al. (2014)
    Anti-leukemic potency of
    piggyBac
    -mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia
    Cytotherapy 16: 1257-1269

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