MultiMACS™ Cell24 Separator Plus

Parallel cell separation of large sample numbers and high sample volumes

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Request a quote Schedule a demo

The MultiMACS Cell24 Separator Plus is a semi-automated cell separator that enables magnetic cell separations based on MACS® MicroBeads and MACS Columns. It is the first step to automated cell separation and allows for parallel multisample magnetic cell isolation using MACS Technology. Whether you are dealing with high sample numbers, large sample volumes, or individual samples, the convenient design of the MultiMACS Cell24 Separator Plus provides versatility to upscale and downscale depending on your requirements. The instrument is designed for isolation of virtually any cell type from any source, giving reliable and standardized results. It is compatible with many downstream applications, such as flow cytometry, cell-culture assays, or molecular analysis.

Benefits Features & specifications Applications Support

Benefits

Speed

Simultaneous cell isolation from large sample volumes, such as buffy coats or Leukopaks®, and high sample numbers, like mouse samples.

Scalability

Parallel processing of one to 24 samples to fit every workflow, including few or small samples and high-throughput applications with the MultiMACS X.

Ease of use

Standard programs for all MACS Cell Separation strategies with step by step instructions that guide you through the separation protocols.

Versatility

Compatible with LS, LD, and Whole Blood columns and Multi-24 Column Blocks for high purity separations by positive selection, depletion, or untouched isolation of practically any target cell.

Features & specifications

MultiMACS Cell24

MultiMACS Magnet and MultiMACS Column Holder

Enable cell separation with up to 12 single columns and the Multi-24 Column Blocks.

Touchscreen

Easy to operate touchscreen interface to guide you through the protocol step by step.

Elution Chamber with Lids

Magnetically labeled cells can be collected in Deep Well Plates or 5 mL tubes.

MACS Elution Station

Vacuum impulse for elution of your labeled cell fraction.

Single-Column Adapter

Parallel isolation of up to 12 samples with LS, LD and Whole Blood Columns.

MultiMACS 5 mL Tube Rack

Collection of your cells in 5 mL tubes.

Multi-24 Column Block and 24-well Deep Well Plate

Isolation of up to 24 samples in parallel and cell collection in plate reservoirs.

1 2 3 4 5 6 7

MultiMACS Magnet and MultiMACS Column Holder

Enable cell separation with up to 12 single columns and the Multi-24 Column Blocks.

Touchscreen

Easy to operate touchscreen interface to guide you through the protocol step by step.

Elution Chamber with Lids

Magnetically labeled cells can be collected in Deep Well Plates or 5 mL tubes.

MACS Elution Station

Vacuum impulse for elution of your labeled cell fraction.

Single-Column Adapter

Parallel isolation of up to 12 samples with LS, LD and Whole Blood Columns.

MultiMACS 5 mL Tube Rack

Collection of your cells in 5 mL tubes.

Multi-24 Column Block and 24-well Deep Well Plate

Isolation of up to 24 samples in parallel and cell collection in plate reservoirs.

MultiMACS Cell24

MultiMACS Cell24 Separator Plus specifications

Principle	Any positive or untouched isolation can be performed using MACS Cell Separation Reagents in combination with the MultiMACS Cell24 Separator Plus. Cells can be isolated from dissociated tissues, single cell-suspensions, PBMCs, or directly from blood products. StraightFrom® MicroBeads together with the MultiMACS Cell24 Separator Plus enable the fastest cell isolation directly from whole blood, buffy coat, LRSC and Leukopak® without density gradient centrifugation. Separations can be performed with the Multi-24 Column Block for simultaneous isolation of up to 24 small samples, or by splitting one large volume sample. Alternatively, up to 12 LS, LD, or Whole Blood Columns can be used in combination with the Single-Column Adapter.
Background	The MultiMACS Cell24 Separator Plus contains a permanent 24-well magnet that induces a high-gradient magnetic field within columns. The field is strong enough to retain cells labeled with even small amounts of MACS MicroBeads in the columns. After transfer of columns to the Elution Chamber of MACS Elution Station, magnetically labeled cells can be eluted from columns via a small vacuum impulse. The touchscreen guides the user through the complete separation process. During cell separation the instrument moves the separation column block into and out of the magnet, thus allowing convenient processing of up to 24 samples in parallel. For elution of the positive fraction, the column block or single columns in the Single-Column Adapter must be transferred to the Elution Chamber. The vacuum-driven elution station ensures gentle elution of positively selected cells for excellent purity and maximal cell viability. The vacuum impulse guarantees simultaneous elution with identical parameters for every sample in every run. Pipetting steps during cell separation procedures are either performed manually with software guidance, or can be fully automated with the MultiMACS X.
Level of automation	Semi-automated
Footprint	MultiMACS Cell24 Separator	Size (w x d x h): 230 mm x 435 mm x 230 mm (9.1" x 17.1" x 9.1"), Weight: 11 kg
Footprint	MACS Elution Station	Size (w x d x h): 114 mm x 265 mm x 233 mm (4.5" x 10.4" x 9.2"), Weight: 4.6 kg
Order number	130-098-637

Column options

Whether following a positive selection or an untouched strategy, parallel separation is fast and provides reproducible results because all samples are treated identically. Different column options enable you to adapt to sample size, type, and separation strategy. The Multi-24 Column Block supports both positive selection and depletion. The Single-Column Adapter is compatible with LS, LD, or Whole Blood Columns. LS Columns are optimized for positive selection and depletion of strongly magnetic labeled cells. LD Columns are optimized for depletion and Whole Blood Columns for cell isolation directly from whole blood, bone marrow, and buffy coat.

Name	Capacity labeled cells (max. magnetically labeled cells)	Capacity total cells (max. total cells or max. blood volume)
Multi-24 Column Block	Up to 1×10⁸ (per single column)	Up to 1×10⁹ (per single column)
LS Column	Up to 1×10⁸	Up to 1×10⁹
LD Column	Up to 1×10⁸	Up to 5×10⁸
Whole Blood Column	NA	Up to 10 mL

Reagent compatibility & starting material

Separation strategies

Positive separation	MACS MicroBeads and MicroBead Kits StraightFrom® MicroBeads MACSprep™ MicroBeads REAlease® MicroBead Kit
Untouched isolation	MACS Isolation Kits
Indirect MicroBeads	e.g. Anti-Biotin MicroBeads

Starting material

Sample type	- PBMCs - Whole blood - Buffy coat - Leukapheresis sample - LRSC - Bone marrow - Single-cell suspensions from dissociated tissue or tumor - Cultured cells (cell lines and primary cells)
Species	- Human - Mouse - Rat - Non-human primate - All others possible with Indirect MicroBeads
Number of samples	- Up to 24 samples with the Multi-24 Column Block - Up to 12 samples with the Single-Column Adapter

Consumables and accessories

Elution and waste plates

Deep Well Plates and 5 mL tubes in a Tube Rack can be used to collect the positive and negative fractions. The Single-well, 2-well, 3-well, 4-well, and 24-well Deep Well Plates are also compatible with the MultiMACS Cell24 Separator Plus and the MultiMACS X. The Deep Well Plate Lids can be used with any plate.

Multi-24 Column Block

The Multi-24 Column Block enables high-throughput and high sample volume applications by combining 24 columns in one block, allowing you to isolate up to 24 samples simultaneously. This saves time and makes processing large sample numbers or volumes more convenient. The Multi-24 Column Block has a capacity of 1x10⁸ labeled cells per column and 1x10⁹ total cells per column.

Multi-24 Column Blocks (8 pieces)
Multi-24 Column Block (1 piece)

Single-Column Adapter

Parallel isolation of up to 12 samples is made possible by the Single-Column Adapter. Designed for use with single MACS Columns, it allows you to handle up to 12 samples in one go with LS, LD, or Whole Blood Columns. Large volumes samples, such as Leukopaks® are divided evenly between the columns and combined at the end of the run to ensure high-quality separations from every sample, regardless of volume.
The desired number of compatible columns is inserted into the holder, while remaining slots are filled with blank columns for elution of the labeled cell fraction with the Elution Chamber. This makes the MultiMACS Cell24 Separator an economic choice also for small sample numbers.

5 mL Tube Rack

5 mL tubes in the Tube Rack can be used to collect the positive and negative fractions. The printing on the MultiMACS 5 mL Tube Rack shows you where to place tubes if using the Single-Column Adapter.

MultiMACS 5 mL Tube Rack

Automation capabilities

The MultiMACS™ X Cell Separator – Fully automated cell separation

View details

The MultiMACS Cell24 Separator Plus is a semi-automated benchtop instrument which performs the cell elution step automatically. Pipetting steps are carried out by the user. Beyond, the MultiMACS Cell24 Separator Plus technology is also at the heart of the fully automated MultiMACS X Separator. The MultiMACS X is a benchtop platform that combines the benefits of the MultiMACS Cell24 Separator Plus and an integrated liquid handling system. Full automation eliminates hands-on steps otherwise performed by the user, such as sample transfer onto the column, washing steps, and buffer pipetting.

Find out more about the MultiMACS X

Videos

Video
MultiMACS Cell24 Separator Plus in action

With a clear goal in mind, the MultiMACS Cell24 Separator Plus was developed to allow automated parallel cell separation. Get a first impression of the instrument and have a look at how it works.

Video
How to separate TILs with the MultiMACS Cell24 Separator

Watch how the MultiMACS Cell24 Separator and the MultiMACS X can enhance your workflow of isolating CD45⁺ tumor-infiltrating leukocytes.

Video
High-throughput magnetic separation of CD11c⁺ DC with the MultiMACS™ Cell24 Separator

CD11c⁺ DCs can be isolated from 24 samples in parallel with the MultiMACS Cell24 Separator Plus in an efficient, yet gentle manner. Find out how!

Video
Zoey and MACS MicroBead Technology

Zoey uses MACS MicroBead Technology to isolate virtually any cell type from any sample material in three easy steps. Learn how MACS MicroBead Technology and the MultiMACS Cell Separator can help you get published.

User manual

MultiMACS Cell24 Separator Plus User Manual

Resources

Brochures/posters

MultiMACS™ Cell Separator Family - Automation meets parallel cell separation

Scientific posters

App notes/customer reports

Selected references

Guito, J. C. et al. (2021) Asymptomatic Infection of Marburg Virus Reservoir Bats Is Explained by a Strategy of Immunoprotective Disease Tolerance. Curr. Biol. 31(2): 257-270
Pascual-García M. et al. (2019)
LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8
⁺
T cell tumorinfiltration impairing anti-PD1 therapy.
Nat Commun. 10: 2416
Ferrara, F. et al. (2018)
Rapid purification of billions of circulating CD19
⁺
B cells directly from leukophoresis samples.
N Biotechnol. 46: 14-21
Crombie, D. et al. (2017) Development of a Modular Automated System for Maintenance and Differentiation of Adherent Human Pluripotent Stem Cells. SLAS Discov. 2(8): 1016-1025
Takeshi, I. et al. (2016) Regulation of CD44 expression and focal adhesion by Golgi phosphatidylinositol 4-phosphate in breast cancer. Cancer Sci. 107(7): 981-990
Paul, D. et al. (2015) Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nat. Methods
Hubner, N. et al. (2011) Extracting gene function from protein–protein interactions using Quantitative BAC InteraCtomics (QUBIC). Methods 53(4): 453-459
Qing-Fang, L. et al. (2008) Sphingosine 1-phosphate induces Mcl-1 upregulation and protects multiple myeloma cells against apoptosis. Biochem. Biophys. Res. Commun. 371(1): 159-162

Applications

Video

Immune cell isolation straight from blood products with the MultiMACS Cell24 Separator

Application note

Automated isolation of CD56⁺ natural killer cells and antibody-dependent cellular cytotoxicity

Application note

Fast and efficient T cell isolation without density gradient centrifugation for CAR T cell engineering

Scientific poster

Workflow automation and parallelization for better analysis of TIL subpopulations

Support

We help you get the best results

Our technical support team is available to assist you throughout your projects including providing advice on experimental design, sample preparation, troubleshooting, and service options for your instrument.
You can access a collection of FAQs and technical documents, including datasheets, protocols, and manuals.
Training at Miltenyi University
Protect your instrument with a comprehensive service contract.

MultiMACS Cell24 Separator Plus

Parallel cell separation of large sample numbers and high sample volumes.
Order no.: 130-098-637.

Request a quote Schedule a demo

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