| Principle | Any positive or untouched isolation can be performed using MACS Cell Separation Reagents in combination with the MultiMACS Cell24 Separator Plus. Cells can be isolated from dissociated tissues, single cell-suspensions, PBMCs, or directly from blood products. StraightFrom® MicroBeads together with the MultiMACS Cell24 Separator Plus enable the fastest cell isolation directly from whole blood, buffy coat, LRSC and Leukopak® without density gradient centrifugation. Separations can be performed with the Multi-24 Column Block for simultaneous isolation of up to 24 small samples, or by splitting one large volume sample. Alternatively, up to 12 LS, LD, or Whole Blood Columns can be used in combination with the Single-Column Adapter. | |
|---|---|---|
| Background | The MultiMACS Cell24 Separator Plus contains a permanent 24-well magnet that induces a high-gradient magnetic field within columns. The field is strong enough to retain cells labeled with even small amounts of MACS MicroBeads in the columns. After transfer of columns to the Elution Chamber of MACS Elution Station, magnetically labeled cells can be eluted from columns via a small vacuum impulse. The touchscreen guides the user through the complete separation process. During cell separation the instrument moves the separation column block into and out of the magnet, thus allowing convenient processing of up to 24 samples in parallel. For elution of the positive fraction, the column block or single columns in the Single-Column Adapter must be transferred to the Elution Chamber. The vacuum-driven elution station ensures gentle elution of positively selected cells for excellent purity and maximal cell viability. The vacuum impulse guarantees simultaneous elution with identical parameters for every sample in every run. Pipetting steps during cell separation procedures are either performed manually with software guidance, or can be fully automated with the MultiMACS X. | |
| Level of automation | Semi-automated | |
| Footprint | MultiMACS Cell24 Separator | Size (w x d x h): 230 mm x 435 mm x 230 mm (9.1" x 17.1" x 9.1"), Weight: 11 kg |
| MACS Elution Station | Size (w x d x h): 114 mm x 265 mm x 233 mm (4.5" x 10.4" x 9.2"), Weight: 4.6 kg | |
| Order number | 130-098-637 | |
Whether following a positive selection or an untouched strategy, parallel separation is fast and provides reproducible results because all samples are treated identically. Different column options enable you to adapt to sample size, type, and separation strategy. The Multi-24 Column Block supports both positive selection and depletion. The Single-Column Adapter is compatible with LS, LD, or Whole Blood Columns. LS Columns are optimized for positive selection and depletion of strongly magnetic labeled cells. LD Columns are optimized for depletion and Whole Blood Columns for cell isolation directly from whole blood, bone marrow, and buffy coat.
| Name | Capacity labeled cells (max. magnetically labeled cells) | Capacity total cells (max. total cells or max. blood volume) |
| Multi-24 Column Block | Up to 1×10⁸ (per single column) | Up to 1×10⁹ (per single column) |
| LS Column | Up to 1×108 | Up to 1×109 |
| LD Column | Up to 1×108 | Up to 5×108 |
| Whole Blood Column | NA | Up to 10 mL |
Separation strategies
| Positive separation | MACS MicroBeads and MicroBead Kits StraightFrom® MicroBeads MACSprep™ MicroBeads REAlease® MicroBead Kit |
|---|---|
| Untouched isolation | MACS Isolation Kits |
| Indirect MicroBeads | e.g. Anti-Biotin MicroBeads |
Starting material
| Sample type | - PBMCs - Whole blood - Buffy coat - Leukapheresis sample - LRSC - Bone marrow - Single-cell suspensions from dissociated tissue or tumor - Cultured cells (cell lines and primary cells) |
|---|---|
| Species | - Human - Mouse - Rat - Non-human primate - All others possible with Indirect MicroBeads |
| Number of samples | - Up to 24 samples with the Multi-24 Column Block - Up to 12 samples with the Single-Column Adapter |
Elution and waste plates
Deep Well Plates and 5 mL tubes in a Tube Rack can be used to collect the positive and negative fractions. The Single-well, 2-well, 3-well, 4-well, and 24-well Deep Well Plates are also compatible with the MultiMACS Cell24 Separator Plus and the MultiMACS X. The Deep Well Plate Lids can be used with any plate.
Multi-24 Column Block
The Multi-24 Column Block enables high-throughput and high sample volume applications by combining 24 columns in one block, allowing you to isolate up to 24 samples simultaneously. This saves time and makes processing large sample numbers or volumes more convenient. The Multi-24 Column Block has a capacity of 1x108 labeled cells per column and 1x109 total cells per column.
- Multi-24 Column Blocks (8 pieces)
- Multi-24 Column Block (1 piece)
Single-Column Adapter
Parallel isolation of up to 12 samples is made possible by the Single-Column Adapter. Designed for use with single MACS Columns, it allows you to handle up to 12 samples in one go with LS, LD, or Whole Blood Columns. Large volumes samples, such as Leukopaks® are divided evenly between the columns and combined at the end of the run to ensure high-quality separations from every sample, regardless of volume.
The desired number of compatible columns is inserted into the holder, while remaining slots are filled with blank columns for elution of the labeled cell fraction with the Elution Chamber. This makes the MultiMACS Cell24 Separator an economic choice also for small sample numbers.
5 mL Tube Rack
5 mL tubes in the Tube Rack can be used to collect the positive and negative fractions. The printing on the MultiMACS 5 mL Tube Rack shows you where to place tubes if using the Single-Column Adapter.
View details
Play video
Brochures/posters
Scientific posters
- Workflow automation and parallelization improves the isolation and analysis of tumor-infiltrating immune cell subpopulations
- Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®
- Tumor-infiltrating T cells: Complete workflows allow faster and improved flow cytometric analysis of syngeneic mouse tumors
- Magnetic enrichment of tumor-infiltrating T cells reduces experimental time and prevents biased functional characterization
- Analysis of T cells made easy – complete workflow improves the isolation and analysis of T cells
App notes/customer reports
- Guito, J. C. et al. (2021) Asymptomatic Infection of Marburg Virus Reservoir Bats Is Explained by a Strategy of Immunoprotective Disease Tolerance. Curr. Biol. 31(2): 257-270
- Pascual-García, M. et al. (2019) Nat Commun. 10: 2416LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+T cell tumorinfiltration impairing anti-PD1 therapy.
- Ferrara, F. et al. (2018) N Biotechnol. 46: 14-21Rapid purification of billions of circulating CD19+B cells directly from leukophoresis samples.
- Crombie, D. et al. (2017) Development of a Modular Automated System for Maintenance and Differentiation of Adherent Human Pluripotent Stem Cells. SLAS Discov. 2(8): 1016-1025
- Takeshi, I. et al. (2016) Regulation of CD44 expression and focal adhesion by Golgi phosphatidylinositol 4-phosphate in breast cancer. Cancer Sci. 107(7): 981-990
- Paul, D. et al. (2015) Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nat. Methods 12(9): 885-892
- Hubner, N. et al. (2011) Extracting gene function from protein–protein interactions using Quantitative BAC InteraCtomics (QUBIC). Methods 53(4): 453-459
- Qing-Fang, L. et al. (2008) Sphingosine 1-phosphate induces Mcl-1 upregulation and protects multiple myeloma cells against apoptosis. Biochem. Biophys. Res. Commun. 371(1): 159-162
We help you get the best results
- Our technical support team is available to assist you throughout your projects including providing advice on experimental design, sample preparation, troubleshooting, and service options for your instrument.
- You can access a collection of FAQs and technical documents, including datasheets, protocols, and manuals.
- Training at Miltenyi University
- Protect your instrument with a comprehensive service contract.
MultiMACS Cell24 Separator Plus
Parallel cell separation of large sample numbers and high sample volumes.
Order no.: 130-098-637.
