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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Viral transduction of CD34+ cells is a promising approach for treating inherited disorders such as sickle cell disease, ß-thalassemia, or primary immunodeficiencies 1,2,3. The CliniMACS Prodigy® Hematopoietic Stem Cell Engineering (HSCE) System allows for manufacturing of gene-engineered hematopoietic stem cells (HSCs) from human CD34+ cells.
Hematopoietic stem cells (human)
The CliniMACS Prodigy Cell Manufacturing Platform automates the generation of gene-engineered HSCs by viral transduction in a functionally closed system. All steps are automated and manufacturing takes place in sterile and single-use tubing sets, ensuring highly reproducible and standardized manufacturing processes.
1. Preparation of CD34+ cells as cellular starting material
The CliniMACS Prodigy HSCE System uses human CD34+ cells as cellular starting material. They can be easily enriched from mobilized leukapheresis with the CliniMACS Prodigy or the CliniMACS Plus Instrument. Enriched CD34+ cells are transferred to a cell bag, which needs to be connected to the CliniMACS Prodigy Tubing Set (TS) 520.
How to enrich CD34+ cells from blood products with CliniMACS Technology?
2. Transduction of HSCs
CD34+ cells are resuspended in HSC-Brew GMP Medium supplemented with MACS® GMP Recombinant Human Cytokines (SCT, TPO, Flt3-Ligand and IL-3) and transduced with lentiviral vector. The transduction step can be repeated, if necessary, to increase transduction efficiency.
3. Harvest of gene-engineered HSCs
After transduction, the final cell product containing gene-engineered HSCs is washed twice and harvested in 100 mL buffer of your choice. The gene-engineered HSCs are then ready for further processing, e.g., cryopreservation, or functional testing.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. For complete regulatory notes, please click here.
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