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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse macrophages are extensively studied in tissues and tumor. For reliable isolation of mouse macrophages, gentle dissociation of lymphoid tissue is key. Mouse spleen can be dissociated into single-cell suspensions using our Spleen Dissociation Kit, mouse in combination with the gentleMACS™ Dissociator with Heaters. Mechanical dissociation with enzymatic degradation of the extracellular matrix maintains the structural integrity of tissues.
MACS Handbook:
Spleen (mouse)
We have developed a fast and efficient way to isolate mouse macrophages based on their specific F4/80 markers by using Anti-F4/80 MicroBeads UltraPure, mouse.
Isolation of F4/80+ macrophages. F4/80+ cells were isolated from spleen single-cell suspension using Anti-F4/80 MicroBeads UltraPure, two MS Columns, and an OctoMACS™ Separator. The cells were fluorescently stained with CD45-VioBlue®, CD11b-APC, and Anti-F4/80-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.
We offer various reagents and kits for the isolation of mouse myeloid cells from tissues and tumors. Download the poster and find a comprehensive overview of all myeloid cell markers and products for convenient isolation and analysis.
Working with MDSCs? The Myeloid-Derived Suppressor Cell Isolation Kit, mouse has been developed for the isolation of CD11b+Gr-1+ myeloid cells from mouse lymphoid tissue.
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Macrophages can be generated in vitro from human monocytes using Human M-CSF, premium grade or Human GM-CSF, premium grade .
MACS Handbook:
Cell culture
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