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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Discover our different mesenchymal stem cell workflows and find the one that fits your experimental needs.
MACS Handbook:
Mesenchymal stem cells (human)
Related PDFs:
Workflow for human mesenchymal stem or stromal cells (brochure)
Stem cell quick guide (brochure)
The MSC Enumeration Kit was developed for the standardized, quick, and absolute quantification of human MSCs from fresh bone marrow aspirate by flow cytometry. It takes only 30 minutes and requires only 200 µl of bone marrow sample per test.
Related PDFs:
MSC Enumeration Kit, human (brochure)
Related products:
Historically, a number of methods relying on specific physical properties have been used to isolate MSCs from sites at which they reside. The problem with this type of approach is that no physical properties have been uniquely ascribed to MSCs. Therefore, many different cell types are co-isolated, resulting in a mixed population of cells.
Magnetic cell isolation (positive selection) of CD271 (LNGFR)+ cells results in a cell population containing an approximately 1000-fold greater concentration of MSCs as compared to conventional methods of isolation. This enables isolation of a homogeneous, and consequently more effective non-hematopoietic stem cell population.
Our cell culture options provide reliable solutions for the maintenance and expansion of MSCs with preserved differentiation potential and immunomodulation ability.
MACS Handbook:
Cell culture
Related PDFs:
StemMACS MSC Expansion Media Kit XF, human (brochure)
StemMACS Mesenchymal Stem Cell Media, human (datasheet)
The MSC Phenotyping Kit was developed for the standardized identification and phenotyping of cultured human MSCs by flow cytometry based on the defined ISCT standards.
Related PDFs:
Related products:
MSC Phenotyping Kit, human
We provide a variety of media options that support the differentiation of MSCs into adipocytes, osteoblasts, and chondrocytes. The different StemMACS Differentiation Media are suitable for the analysis or quality control of the differentiation capacity of expanded MSCs, as well as in vitro studies focusing on the processes involved in MSC differentiation, including gene expression and protein profiling.
Related PDFs:
StemMACS Mesenchymal Stem Cell Media, human (datasheet)
The suppression potential of MSCs varies among in vitro expanded cells and shows donor-dependent differences. MSCs can be “licensed” by inflammatory cytokines such IFN-γ and TNF-α to become more immunosuppressive and show a more homogeneous phenotype in this regard. The MSC Suppression Inspector, human was developed for standardized characterization of the immunomodulatory function of MSCs by co-culture with CD4+CD25– or CD4+ responder T (Tresp) cells
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