Bone marrow- and adipose tissue-derived mesenchymal stem cells (MSCs)

The MSC Enumeration Kit was developed for the standardized, quick, and absolute quantification of human MSCs from fresh bone marrow aspirate by flow cytometry.  It takes only 30 minutes and requires only 200 µl of bone marrow sample per test. 

Historically, a number of methods relying on specific physical properties have been used to isolate MSCs from sites at which they reside. The problem with this type of approach is that no physical properties have been uniquely ascribed to MSCs. Therefore, many different cell types are co-isolated, resulting in a mixed population of cells.

Magnetic cell isolation (positive selection) of CD271 (LNGFR)⁺ cells results in a cell population containing an approximately 1000-fold greater concentration of MSCs as compared to conventional methods of isolation. This enables isolation of a homogeneous, and consequently more effective non-hematopoietic stem cell population.

Our cell culture options provide reliable solutions for the maintenance and expansion of MSCs with preserved differentiation potential and immunomodulation ability.

The MSC Phenotyping Kit was developed for the standardized identification and phenotyping of cultured human MSCs by flow cytometry based on the defined ISCT standards.

The suppression potential of MSCs varies among in vitro expanded cells and shows donor-dependent differences. MSCs can be “licensed” by inflammatory cytokines such IFN-γ and TNF-α to become more immunosuppressive and show a more homogeneous phenotype in this regard. The MSC Suppression Inspector, human was developed for standardized characterization of the immunomodulatory function of MSCs by co-culture with CD4⁺CD25^– or CD4⁺ responder T (Tresp) cells.