Isolation of monocytes and generation of  monocyte derived dendritic cells

Application protocols

Application data by workflow step

Increasing number of studies focus on the distinct functions of classical an non-classical monocyte subsets. We have developed specific kits for the separation of monocytes and monocyte subsets.

Using our new StraightFrom™ Portfolio we provide tools for fast and easy isolation of monocytes directly from whole blood, buffy coat, and leukapheresis without the need for density gradient centrifugation,  wash steps, erythrocyte lysis, and cell counting. Using StraightFrom™ Buffy Coat CD14 MicroBead Kit, human you can get pure CD14⁺ monocytes directly from buffy coat in less than 30 minutes.

Generation of Mo-DCs 

Large numbers of DCs for basic research and immunotherapies can be generated in vitro from monocytes using several differentiation/maturation protocols. To obtain functional Mo-DCs that activate T cells efficiently, reliable procedures using high-quality reagents are necessary.