This workflow is optimized for easy and fast isolation of CD14+ monocytes and generation of monocyte-derived DCs (Mo-DCs). Plus, it enables translation of your Mo-DC research for clinical purposes.
Highly pure CD14+ monocytes
CD14 MicroBeads enable the isolation of viable CD14+ monocytes to high purities, with high yields. The isolated monocytes are suited for subsequent differentiation into Mo-DCs.
Using our new StraightFrom™ Portfolio we provide tools for fast and easy isolation of monocytes directly from whole blood, buffy coat, and leukapheresis without the need for density gradient centrifugation, wash steps, erythrocyte lysis, and cell counting. Using StraightFrom™ Buffy Coat CD14 MicroBead Kit, human you can get pure CD14+ monocytes directly from buffy coat in less than 30 minutes.
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Analysis gate
CD14+ cells
Isolation of CD14+ monocytes from buffy coat. Separation of a buffy coat sample using the StraightFrom™ Buffy Coat CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD15-APC, CD14-PE, as well as CD45‑VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Large numbers of DCs for basic research and immunotherapies can be generated in vitro from monocytes using several differentiation/maturation protocols. To obtain functional Mo-DCs that activate T cells efficiently, reliable procedures using high-quality reagents are necessary.
Scientific Poster
Monocyte-derived dendritic cells (Mo-DCs) generated with CliniMACS Prodigy® are functional and fulfill requirements for cancer vaccines
Mareke Brüning, Tobias Ozimkowski, Eva Bergschneider, Michael Birth, Stefan Wild, and Andrzej Dzionek
Webinar
Isolation and expansion of MSCs for translational and clinical research
Learn in this webinar about MSC expansion, the CliniMACS Prodigy® Adherent Cell Culture System to acquire GMP-compliant and clinical-scale MSCs, and fast and easy characterization of MSCs via flow cytometry.
MBTP 13: Immunophenotyping of monocytes from mouse spleen using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- monocytes
- Antibody panel
- Application protocol
Multicolor flow cytometric analysis of monocytes from mouse spleen
- Keywords
- Sample preparation
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- monocytes
- Application protocol
Generation of Mo-DCs
- Keywords
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- human
- PBMCs
- monocytes
- dendritic cells
- Application protocol
