Vio® Bright R720 and Vio® R720 fluorophores

Absorption and emission spectra of Vio Bright R720 and Vio R720

• Suitable for all instrument equipped with a red laser (640 nm) and a standard 725/40 nm filter set
• Similar absorption and emission spectra to Alexa Fluor 700 and APC-R700

Brighter and clearer staining for red laser

• 2-fold or higher increase in brightness compared to Alexa Fluor 700
• Increase in brightness of Vio Bright R720 conjugates across various antigens, e.g., CD19 or CD56
• Higher mean fluorescent intensity (MFI) and stain index (SI) compared to Alexa Fluor 700

Decreased background noise

• Lysed whole blood may results in unspecific background noise. Therefore it is important to avoid additional unspecific binding
• Vio Bright R720 conjugates show decreased background noise compared to Alexa Fluor 700 and APC-R700

Excellent performance with different staining protocols

• Excellent stability after fixation with paraformaldehyde
• Compatible with different surface and intracellular staining protocols and buffer sets
• Conjugate performance may vary depending on antigen nature upon fixation

High photostability

• No major changes in photostability after 24 hours or exposure to ambient light (~850 lux)

Fluorescence spectra viewer 

Check out our fluorescence spectra viewer for well-planned multicolor flow cytometry experiments.