Magnetic immune cell subset isolation for virology research

Isolated immune cell subsets can be used to study T cell immune response to a viral infection, isolate B cells from immunized animals in vaccine research, or monitor cell-mediated immunity in recovered patients. Of course, assays are only as reliable as their components, hence the need for highly pure and viable cell subsets for co-stimulation assays, killing assays, and genomic analyses. Miltenyi Biotec offers magnetic cell isolation products for direct as well as untouched immune cell isolation from human, mouse, and non-human primate-derived tissues and blood products.  

Isolating cell subsets from tissue samples can be complex, but combining the gentleMACS™ Dissociator with MACS MicroBeads gives unmatched purity and viability, which can be further enhanced using the Dead Cell Removal Kit.  

Indirect MicroBeads, such as anti-biotin MicroBeads, are useful when working with novel markers for which direct cell isolation products are not available.  

Minimizing exposure to potentially contaminated samples is critical for scientists’ safety, and fully automated workflows allow precious time in the lab to be spent on other tasks. 
 

For high-throughput cell isolations we recommend the MultiMACS™ Instrument Family, which allows magnetic cell isolation from up to 24 samples simultaneously, as well as processing of large blood samples, such as buffy coats, without the use of density gradient centrifugation. 
 

• Structural and functional analysis of a potent sarbecovirus neutralizing antibody; Pinto et al. (2020) BioRxiv.
• CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice; Veit et al. (2018) Viruses 16:10(12); (PMID: 30558354)
• The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression; Grunewald, M. E. et al. (2019) PLOS Pathogens;  (PMID: 31095648)
• Antibody-dependent enhancement of SARS coronavirus infection and its role in the pathogenesis of SARS; Yip, M. S. et al. (2016) Hong Kong Med. J. 22: (3 Suppl. 4): 25–31; (PMID: 27390007)
• The MHC class-II HLA-DR receptor mediates bat influenza A-like H17N10 virus entry into mammalian cells;  Giotis, E. S. et al. (2019); (PMID: 31358984)
• Requirement for memory B-cell activation in protection from heterologous influenza virus reinfection; Leach, S. et al. (2019) Int. Immunol. 31(12): 771–779; (PMID: 31231764)
• Single dose combination nanovaccine provides protection against influenza A virus in young and aged mice; Ross, K. et al. (2019) Biomater Sci. 7(3): 809–821; (PMID: 30663733)
• Measles Vaccines Designed for Enhanced CD8+ T Cell Activation; Busch, E. et al. (2020) Viruses 12(2); (PMID: 32098134)
• Prophylactic and Postexposure Efficacy of a Potent Human Monoclonal Antibody Against MERS Coronavirus; Corti et al. (2015) PNAS 112(33):10473-8; (PMID: 26216974)