B cell malignancies research

Application notes, protocols and scientific poster for B cell malignancies

• Increased sensitivity of flow cytometry for detection of minimal residual disease and circulating tumor cells in B Non-Hodgkin Lymphoma (Application protocol)
• A standardized and reproducible method for expansion and activation of human primary B cells (Scientific poster)
• Sensitive analysis and isolation of ROR1⁺ B cells for research on chronic lymphocytic leukemia (Application note)

B cell separation

Whether you require highly purified populations separated by positive selection or untouched cells from whole blood, MACS Technology has a solution for you.

For the isolation of ROR1⁺ B cells, we have developed the Anti-ROR1 MicroBead Kit, human. Using this kit, ROR1⁺ cells are indirectly magnetically labeled with an Anti-ROR1-PE antibody and Anti-PE MicroBeads. The isolated ROR1⁺ B cells are then ready for any downstream analysis. ROR1⁺ cells can be enriched to high purities and numbers suitable for further research processing – even from samples with very low ROR1⁺ B cell frequencies, e.g., in minimal residual disease (MRD).

MACSxpress® Technology is designed to isolate viable and functional B cells fast and conveniently from blood and blood products. This cell isolation technology is based on micro-sized MACSxpress Beads, which allow the removal of non-target cells by immunomagnetic depletion using the MACSxpress Separator, a powerful permanent magnet. All without the need of density gradient centrifugation or red blood cell lysis. 

Multiparameter flow analysis of malignant B cells

As the final step in this workflow, flow analysis plays an important part. The following flow reagents can help you make sure that the data you generate on your flow cytometer are reliable and consistent: 

• REAfinity™ Recombinant Antibodies – recombinantly generated antibodies that ensure lot-to-lot consistency and eliminate background signal
• MACSplex™ Cytokine Kits – analysis of multiple soluble cytokines in one sample using any standard flow cytometer
• Cytokine Secretion Assay – Detection Kits – flow analysis of cytokine secretion at a detection level of one in a million
• Build your own B cell flow panel with our Flow Panel Builder

Reproducibility is a key factor in flow cytometry experiments. It can be influenced by biological material, reagents and instrumentation, as well as analysis of acquired data, whereby the operator leads to variable results. 
Our MACSQuantify™ Express Modes are software add-ons for our MACSQuant® Analyzer Flow Cytometers. They are algorithm-based gating tools that allow for an unbiased and user-independent analysis, resulting in improved reproducibility of flow cytometry data and statistics. Based on real data, analysis and acquisition, scripts facilitate automated, application-specific gating on the actual population distribution.