B cell malignancies research

Isolation of ROR1⁺ B cells 

For the isolation of ROR1⁺ B cells, we have developed the Anti-ROR1 MicroBead Kit, human. Using this kit, ROR1⁺ cells are indirectly magnetically labeled with an Anti-ROR1-PE antibody and Anti-PE MicroBeads. The isolated ROR1⁺ B cells are then ready for any downstream analysis. ROR1⁺ cells can be enriched to high purities and numbers suitable for further research processing – even from samples with very low ROR1⁺ B cell frequencies, e.g., in minimal residual disease (MRD).

Enrichment of ROR1⁺ cells

Enrichment of ROR1⁺ cells from PBMCs of a donor with MRD. ROR1⁺ cells were isolated using the Anti-ROR1 MicroBead Kit, either manually. Cells were fluorescently labeled with Anti-ROR1-PE and CD19-APC and analyzed by flow cytometry. Purities of the manually and automatically isolated cells amounted to 94% and 98%, and yields amounted to 45% and 97%, respectively.

As the final step in this workflow, flow analysis plays an important part. The following flow reagents can help you make sure that the data you generate on your flow cytometer are reliable and consistent: 

• REAfinity™ Recombinant Antibodies – recombinantly generated antibodies that ensure lot-to-lot consistency and eliminate background signal
• MACSplex™ Cytokine Kits – analysis of multiple soluble cytokines in one sample using any standard flow cytometer
• Cytokine Secretion Assay – Detection Kits – flow analysis of cytokine secretion at a detection level of one in a million
• Build your own B cell flow panel with our Flow Panel Builder

Flow cytometric analysis of ROR1⁺ cells and other immune cells in PBMCs from a CLL sample

Identification of ROR1⁺ cells and other immune cells in PBMCs from a CLL sample. Cells were labeled with CD20-VioBlue, CD43-FITC, CD5-PE, CD79b-PerCP-Vio700, CD19-PE-Vio770, Anti-ROR1APC, and CD3-APC-Vio770, and analyzed using the MACSQuant Analyzer 10. CD19⁺ROR1⁺ B cells are shown in magenta, CD19⁺ROR1^– B cells in light blue, T cells in yellow, and NK cells in black.