B cells, through their ability to produce specific antibodies, are essential to control invading viruses and play a primary role in the adaptive immune defense.
Isolation and analysis of B cells and antibodies from SARS-CoV-2 infected or immunized individuals is important to understand the immune response against SARS-CoV-2 infection and for the evaluation of the efficacy of new vaccines.
Screening SARS-CoV-2-specific B cells can support the evaluation of efficacy of COVID-19 vaccines and also the prognosis of severe COVID-19 development. The SARS-CoV-2 B Cell Analysis Kit, human offers an easy and ready-to-use solution for detection, quantification and phenotyping of spike- and RBD-specific B cells circulating in peripheral blood.
Find out more details in our online application protocol: Analysis of SARS-CoV-2–specific B cells using recombinant SARS-CoV-2 proteins
Positive selection of SARS-CoV-2-specific B cells using the SARS-CoV-2 B Cell MicroBead Kit, human, facilitates the enrichment of B cells specific for spike proteins or RBD.
The several-fold enriched antigen-specific B cells can be used for:
Find out more details in our online application protocol: Enrichment of SARS-CoV-2–specific B cells using recombinant SARS-CoV-2 proteins
After staining with SARS-CoV-2 B Cell Analysis Kit, human antigen-specific B cells, recognizing SARS-CoV-2 antigens, can be sorted using the MACSQuant Tyto Cell Sorter. These can be then used for further applications, including further expansion in culture, molecular analysis and single clone culture.
Find out more details in our online application protocol: Microchip-based cell sorting and expansion of SARS-CoV-2–specific B cells using the MACSQuant® Tyto® Cell Sorter
Sorting of antigen specific B cells using the MACSQuant® Tyto®
After enrichment SARS-CoV-2-specific B cells can be cultured using B Cell Expansion Kit, human.
The combination of CD40L, cross-linking antibody, and IL-4 included in the B Cell Expansion Kit allows you to expand the B cell population by several fold and obtain activated cells with a distribution of memory and plasma cells similar to the original fraction.
After expanding SARS-CoV-2-specific B cells, SARS-CoV-2-specific IgG antibodies can be detected in the supernatant, confirming that the B Cell Expansion Kit supports the expansion of fully functional antibody-secreting cells, suitable for applications such as monoclonal antibody discovery and generation.