Reliable analysis of rare virus-specific T cells 

T cells play a pivotal role in fighting viral infections and generating long-term protective immunity after infection and vaccination. 

Assessment of specificity, phenotype, and cytokine secretion of rare virus-reactive T cells can provide essential information on infection progression, prior infections or vaccination efficacy. 

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SARS-CoV-2 T Cell Analysis Kits

All-in-one SARS-CoV-2 T Cell Analysis Kits to monitor and analyze human SARS-CoV-2-reactive T cell subsets from PBMC or whole blood samples. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator of choice and an optimized flow panel (see table below).

SARS-CoV-2 T Cell Analysis Kits

A complete workflow for stimulation, enrichment and analysis of virus-reactive T cells

Untouched isolation of PBMCs from human whole blood

Successful experiments always start with efficient sample preparation. 

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Virus-specific in vitro stimulation of T cells and other immune cells 

Our PepTivator® Peptide Pools enable efficient antigen-specific stimulation of both CD4+ and CD8+ T cells, as well as recombinant SARS-CoV-2 proteins for other immune cells. 

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Application note 

Fast and efficient detection of SARS-CoV-2-reactive T cells via antigen-specific stimulation

See how we used our new SARS-CoV-2 T Cell Analysis Kit (PBMC), human for the stimulation and analysis of virus-reactive T cells in the context of COVID-19.

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Enrichment and sorting of antigen- and virus-specific T cells

Antigen- and virus-specific T cells are extremely which makes analysis difficult. Our optimized enrichment and sorting solutions enable you to even analyze the rarest cells.

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Protocol

Rapid antigen-specific T cell enrichment (Rapid ARTE)

Combine magnetic cell enrichment and multiparameter flow cytometry analysis for direct ex vivo characterization of human antigen-specific CD154+CD4+ T cells.

Safe flow sorting of antigen- and virus-specific T cells

When working with contaminated or infectious material, operator safety is of the utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of even the rarest virus-specific T cells in a closed aerosol-free cartridge system.

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Video

MACSQuant Tyto: Cell sorting as easy as it can be

Find out more about the easy cell sorting procedure on the MACSQuant Tyto: It's simple, it's gentle, it's sterile. This video will give you an overview of the straightforward operator steps from sample loading to retrieval.

Multiparameter flow analysis of antigen- and virus-specific T cells

Our MACS® Flow Cytometry kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility and most precise results. The MACSQuant Analyzer family with automated MACSExpress® Analyses modes eliminate operator variability and standardize your analysis further.

  • SARS-CoV-2 T Cell Analysis Kits – true all-in-one kits for the efficient stimulation and analysis of human SARS-CoV-2-reactive T cells. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator of choice and an optimized flow panel (see table below)
  • REAfinity™ Recombinant Antibodies - recombinantly engineered for higher purity, superior lot-to-lot consistency, and lowest background.
  • MACSplex Cytokine Kits for a multiplexed analysis of secreted cytokines in cell culture supernatant.
  • Rapid Cytokine Inspector Kits - unique for the fastest combination of surface marker and intracellular cytokine staining.
  • 8-Color Immunophenotyping Kit, human for reliable identification of leukocyte subsets in one simple kit, e.g. monocytes, neutrophils, eosinophils, and T, B, and NK cell populations
  • MACSQuant® Analyzers - Fully automated benchtop flow cytometers for highly sensitive, multiparameter flow analyses.

Optimized flow panel for SARS-CoV-2 T Cell Analysis
 

MarkerCloneFluorophore 
CD3REA613 APC 
CD4REA623 Vio® Bright B515
CD8REA734 VioGreen™
CD154REA238APC-Vio® 770
IFN-g REA600PE 
IL-2REA689PE-Vio® 615
TNF-a REA656PE-Vio® 770
CD14REA599 VioBlue®
CD20REA780 VioBlue 
Live/dead Viobility™ Fixable Dye405/452 

Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.

*For VioDye spectra see here

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Check out more supporting materials on antigen-specific T cells