Retroviral transduction of hematopoietic stem cells (HSCs)

MACS® MicroBead Technology provides a broad range of marker-specific, magnetic cell enrichment solutions applicable for stem cell isolation. CD34 is a well-established marker of human hematopoietic stem and progenitor cells, which can be targeted for magnetic enrichment of this cell type. The CD34 MicroBead Kit UltraPure contains MicroBeads directly conjugated to CD34 antibodies for magnetic labeling of CD34-expressing cells from peripheral blood, cord blood, bone marrow, or apheresis harvest.

StemMACS™ HSC Expansion Media XF enables robust expansion of hematopoietic stem cells from cord blood, bone marrow, or peripheral blood. Combined with the StemMACS HSC Expansion Cocktail, a set of cytokines, this xeno-free culture medium can be used to preserve high levels of primitive CD34⁺CD133⁺ hematopoietic cells.

Flow cytometric analyses provide you with quantitative information about the expression of intracellular as well as surface markers and let you assess the efficiency and quality of your viral transduction in just a few simple steps.