Vio® Bright V423 fluorophore 

Compared to polymer-based fluorochromes with high fluorescence intensities, such as Brilliant Violet 421 and Super Bright 436, Vio Bright V423 exhibits a similar or brighter degree of fluorescence. Increased brightness of Vio Bright V423  conjugates is observed across different antigens; the data below show examples of CD19 and CD56 staining. 

Vio Bright V423 requires only minimal compensation

Compared to the other dyes excited by violet lasers, Vio Bright V423 shows minimal spillover into the neighboring detection channels. Thus, with a minimal change in compensation settings, Vio Bright V423 provides the benefit of a brighter dye. 

Multicolor flow cytometry panels

Miltenyi Biotec–tested panels (MBTPs) are a collection of multicolor panels that are optimized and pre-designed for specific flow cytometry applications. They are developed and validated by Miltenyi Biotec’s R&D experts, as well as by the customers who actually use Miltenyi Biotec antibodies. 

• MBTP 20: Immunophenotyping of the activation status of NK cells from human PBMCs using flow cytometry 
• MBTP 32: Immunophenotyping of human γδ T cells using flow cytometry