Towards the identification of virus-specific antibodies 

Plasma cells, through their ability to produce specific antibodies, are essential to control invading viruses and play a primary role in the adaptive immune defense. Isolation of plasma cells from virus-infected individuals can serve as a first step towards the identification of virus-specific antibodies. 

StraightFrom® Whole Blood CD138 MicroBeads allow for the positive selection of CD138+ plasma cells directly from whole blood.

 The isolation procedure is fast with minimized hands-on time; it can be automated and does not require prior density gradient centrifugation and erythrocyte lysis. 

Before isolation
  After isolation

Separation of CD138+ cells from a whole blood sample spiked with U266 cells, using the StraightFrom® Whole Blood and Bone Marrow CD138 MicroBeads. Cells were stained with CD45-FITC, CD138-PE, and CD19-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, dead cells, and CD45 cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Beyond plasma cells, isolation of memory B cells, and total B cells from various starting materials, please check out our related products below.

 

Check out our complete B cell portfolio