Pluripotent stem cell (PSC) differentiation into cardiomyocytes 

StemMACS CardioDiff Kit XF, human has been designed to ease iPSC differentiation to cardiomyocytes in just eight days.

This ready-to-use and xeno-free cell culture system ensures high differentiation efficiency with no additional patterning factors, providing you an easy to follow protocol to maintain strong experiment-to-experiment consistency.

hPSC-derived cardiomyocytes can be further maintained with StemMACS Cardiac Cultivation Medium XF, human for up to 30 days. If cells need to be stored for a longer time, they should be cryopreserved in StemMACS Cryo-Brew to maintain their integrity.

After thawing, cardiomyocytes should be cultured in StemMACS Cardiac Cultivation Medium XF, human to ensure a fast recovery. In both cases, hPSC-derived cardiomyocytes keep on expressing characteristic markers and show typical morphology.

PSC-derived cardiomyocytes comprise a variety of subtypes or subpopulations, such as atrial-like, ventricular-like, and pacemaker-like cells. Characterization has been traditionally done by lengthy and difficult electrophysiological analysis of the respective cell cultures. By contrast, adopting a flow-cytometric characterization approach provides a simpler and time-saving alternative for qualitative and quantitative analysis. 

Our flow cytometry panel for the characterization of PSC-derived cardiomyocyte populations is based on novel recombinantly engineered cardiomyocyte-specific REAfinity™ Antibodies that additionally allow the detection of ventricular and atrial subpopulations.