Optimized tissue sample storage for genomic analysis

Tissue sample storage is necessary when you need to collect and store samples until ready for further processing or shipping to collaboration sites. Especially for genomic analyses, ensuring the right conditions for tissue sample storage are key to avoid changes in cellular composition and minimize cellular stress responses that could negatively impact downstream analyses. The MACS Tissue Storage Solution ensures to uphold tissue quality and integrity to get reliable genomic results in next generation sequencing and single-cell sequencing.

 

Genomic analysis of stored solid tissues

Gene expression analysis of stress-related genes
Gene expression analysis of stress-related genes in stored tumors compared to fresh tumors shows no changes in expression levels

Prevention of cellular stress responses after short-term storage of mouse tumors

After 48 hours of storage in MACS Tissue Storage Solution, mouse tumors showed minimal changes in cellular stress-related gene expression as compared to fresh tumors. This is an advantage to increasing work-time flexibility in a genomics lab and for reliable downstream gene expression analyses.

Required products

MACS Tissue Storage Solution

Related PDFs

Optimal storage of murine tumors in MACS® Tissue Storage Solution (application note)
 

How to store tissue samples using MACS Tissue Storage Solution

Tissue sample storage in MACS Tissue Storage Solution enables flexibility in sample processing by allowing the optimal storage of solid tissues for at most 48 h at 2–8°C. It has been validated with a variety of human and murine tissues including tumor, lung, spleen, brain, or skeletal muscle. 

Preserved tumor cell viability after 24 and 48 h storage time in MACS Tissue Storage Solution
Preserved tumor cell viability after 24 and 48 h storage time in MACS Tissue Storage Solution

Optimal storage of murine tumors up to 48 h

Freshly extracted tumor tissue has been validated for storage up to 48 h in MACS Tissue Storage Solution without compromising cell viability or immune cell composition.

Preservation of TIL composition and activation status
Analysis of tumor tissue after storage shows TIL composition was preserved

Preservation of TIL composition and activation status

Flow cytometric analysis of TIL populations after storage in MACS Tissue Storage Solution show preservation of TIL phenotype and composition. Uncompromised immune cell composition allows for reliable results in further analyses. 

Required products

MACS Tissue Storage Solution 
Tumor Dissociation Kit, mouse 
gentleMACS Octo Dissociator with Heaters 
MACS Smart Strainers (70µm) 

Related videos

How to dissociate tumor tissue (mouse) with the gentleMACS Dissociator

MACS Handbook chapters

Sample preparation
Tumor tissue (mouse)

Further information

Workflow overview

The complete workflow for cell and nuclei preparation for genomics
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Webinar

Preparation of cells and nuclei for genomic analysis
Instrument information

gentleMACS Octo Dissociator with Heaters

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