Name: CD137 MicroBead Kit, human
Availability: InStock

Product data and images for CD137 MicroBead Kit, human

Figures

Figure 1

PBMCs of a CMV

⁺

donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137

⁺

T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Stimulated sample

Unseparated fraction	After separation
View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.	View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
Unstimulated control

Unseparated fraction	After separation
View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.	View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Specifications for CD137 MicroBead Kit, human

Overview

The CD137 MicroBead Kit was developed for the positive selection of human CD137

⁺

cells from PBMCs.

Detailed product information

Background information

CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4

⁺

and CD8

⁺

T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4

⁺

or CD8

⁺

T cells, and NK cells.

Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8

⁺

T cells, as CD137 is not expressed on resting CD8

⁺

T cells and its expression is reliably induced after 24 hours of stimulation.

CD137

⁺

cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. For the isolation of activated antigen-specific CD8

⁺

T cells, the CD137 MicroBead Kit can be combined with the CD8

⁺

T Cell Isolation Kit.

Downstream applications

In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.

The CD137 MicroBead Kit was developed for positive selection of CD137

⁺

cells for phenotypical and functional characterization of activated CD137

⁺

T cells.

Columns

MS, LS, or autoMACS

^®

Columns.

Resources for CD137 MicroBead Kit, human

Documents and Protocols

App notes/customer reports

Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets. Bacher, P. & Scheffold, A. (2015)

References for CD137 MicroBead Kit, human

Publications

Wolfl, M. et al. (2007)
Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8
⁺
T cells responding to antigen without requiring knowledge of epitope specificities.
Blood 110: 201-210
Wehler, T. C. et al. (2007) Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines. Blood 109: 365-373
Schmied, S. et al. (2015)
Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4
⁺
and CD8
⁺
memory formation.
Immunology 145(4): 558-569
Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928

Data and images

Data and images for CD137 MicroBead Kit, human

Figures

Figure 1

PBMCs of a CMV

⁺

donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137

⁺

T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Stimulated sample

Unseparated fraction	After separation
View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.	View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.
Unstimulated control

Unseparated fraction	After separation
View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.	View details Figure 1 PBMCs of a CMV ⁺ donor were stimulated overnight with PepTivator™ CMV pp65 or left untreated. The responding CD137 ⁺ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC.

Specifications

Specifications for CD137 MicroBead Kit, human

Overview

The CD137 MicroBead Kit was developed for the positive selection of human CD137

⁺

cells from PBMCs.

Detailed product information

Background information

CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4

⁺

and CD8

⁺

or CD8

⁺

T cells, and NK cells.

Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8

⁺

T cells, as CD137 is not expressed on resting CD8

⁺

T cells and its expression is reliably induced after 24 hours of stimulation.

CD137

⁺

cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. For the isolation of activated antigen-specific CD8

⁺

T cells, the CD137 MicroBead Kit can be combined with the CD8

⁺

T Cell Isolation Kit.

Downstream applications

In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.

The CD137 MicroBead Kit was developed for positive selection of CD137

⁺

cells for phenotypical and functional characterization of activated CD137

⁺

T cells.

Columns

MS, LS, or autoMACS

^®

Columns.

Resources

Resources for CD137 MicroBead Kit, human

Documents and Protocols

App notes/customer reports

Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets. Bacher, P. & Scheffold, A. (2015)

References

References for CD137 MicroBead Kit, human

Publications

Wolfl, M. et al. (2007)
Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8
⁺
T cells responding to antigen without requiring knowledge of epitope specificities.
Blood 110: 201-210
Wehler, T. C. et al. (2007) Targeting the activation-induced antigen CD137 can selectively deplete alloreactive T cells from antileukemic and antitumor donor T-cell lines. Blood 109: 365-373
Schmied, S. et al. (2015)
Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4
⁺
and CD8
⁺
memory formation.
Immunology 145(4): 558-569
Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928

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CD137 MicroBead Kit, human

CD137 MicroBead Kit, human

Product overview

Product data and images for CD137 MicroBead Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for CD137 MicroBead Kit, human

Overview

Detailed product information

Background information

Downstream applications

Columns

Resources for CD137 MicroBead Kit, human

Documents and Protocols

References for CD137 MicroBead Kit, human

Publications

Data and images for CD137 MicroBead Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for CD137 MicroBead Kit, human

Overview

Detailed product information

Background information

Downstream applications

Columns

Resources for CD137 MicroBead Kit, human

Documents and Protocols

References for CD137 MicroBead Kit, human

Publications

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