Tissue clearing protocols

Optimal immunostaining and clearing of tissues for 3D imaging analysis.


How to clear a mouse brain for 3D imaging analysis

Discover with this video protocol how to easily perform immunostaining and clearing of an adult mouse brain using our MACS® Clearing Kit.

Sample preparation for 3D imaging requires the optical clearing and immunostaining of opaque tissues. Because biological tissues have different molecular compositions, protocols for tissue clearing need to be adjusted to enable efficient 3D imaging analysis of each particular tissue. 

Using our MACS Clearing Kit, we have developed step-by-step protocols with detailed descriptions on how to perform immunostaining and clearing of different tissues for optimal 3D imaging analysis. Each protocol has been optimized to allow the fast immunostaining and clearing of each specific tissue in an easy way. Discover how these protocols can help you to get the best results from your 3D imaging analysis!  

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