MACSPlex Mix - Multiplex assays - Kits and support reagents - MACS Flow Cytometry - Products - Miltenyi Biotec

How to assemble your MACSPlex Mix?

Our MACSPlex Mix products allow you to combine individual analytes within one application category according to your needs. This option is available for the MACSPlex Cytokine Kit, human, and the MACSPlex Cytokine Kit, mouse, but will be extended soon.

To put together your MACSPlex Mix, you need the following components:

MACSPlex Cytokine Basic Kit, human and mouse
MACSPlex Cytokine 12 Standard, human or MACSPlex Cytokine 10 Standard, mouse
and a combination of up to 7 individually available MACSPlex Reagent Kits (see product list below) belonging to one application category (either human or mouse)

Available options

Because each single reagent has a specific fluorescence property, the combination of analytes is only possible within one defined application category. After choosing your first analyte, compatible reagents will be visible by clicking on the product name on the product page. Human and mouse specificities cannot be mixed within a single experiment.

Overview of combinable reagents and necessary software versions to run Express Modes

Application category	Number of analytes	Available target analytes	MACSQuantify Software version	Express Mode package
Human cytokines	Up to 7 analytes of choice	IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α	2.8 and higher	28.1.15443 and higher
Mouse cytokines	Up to 7 analytes of choice	IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α	2.8 and higher	28.1.15443 and higher

Basic principle

Principle of MACSPlex assays

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MACSPlex assays are based on the fluorescent detection of MACSPlex (MPx) Capture Beads, which display defined fluorescence properties that can be identified using standard flow cytometry techniques.

MPx Capture Beads contain a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody that reacts to one analyte.

For some kits a labeling step with biotin-conjugated antibodies is added to enhance signal intensity. Finally, the detection reagent leads to a signal that is measurable by flow cytometry.

Please find kit-specific adaptations of the technology in the gallery tab of the respective product page.

Prerequisites

For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.

Analysis with MACSQuant Analyzers and Express Modes

Exemplary analysis and results using a MACSPlex Cytokine Kit

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When using the MACSQuant Analyzer 10 or MACSQuant X, our MACSplex Kits can be combined with fully automated Express Modes.

By this, an automated gating strategy to populations of interest will be applied and the gates will be automatically adjusted for each data file individually to achieve optimal results.

Furthermore, a one-click batch analysis of all samples belonging to one experiment can be performed to directly compare your data. The concentrations of each analyte will be presented as an exportale table as well as a ready-to-publish graph.

ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.

Analysis with other instruments

For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays.

30-day trial versions are available for both Flowlogic Software and the Beadlogic Add-on.

Additionally, third-party software can be used for analysis.