Mix and match singleplex reagents

High-parameter measurements with up to 7 specificities at the same time
Perfect match with MACSQuant® Analyzers and Express Modes for fully automated experiment setup, acquisition, and analysis
Time-saving method compared to other flow cytometry-based multiplex assays

How to assemble your MACSPlex Mix?

Our MACSPlex Mix products allow you to combine individual analytes within one application category according to your needs. This option is available for the MACSPlex Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human, and the MACSPlex Cytokine Kit, mouse.

To put together your MACSPlex Mix, you need the following components:

MACSPlex Cytokine Basic Kit, human and mouse or MACSPlex Mix Cytotoxic Basic Kit
MACSPlex Cytokine 12 Standard, human, MACSPlex Mix Cytotoxic Standard or MACSPlex Cytokine 10 Standard, mouse
and a combination of up to 7 individually available MACSPlex Reagent Kits (see product list below) belonging to one application category (either Human Cytokine , Human Cytotoxic T/NK or Mouse Cytokine)

Available options

Because each single reagent has a specific fluorescence property, the combination of analytes is only possible within one defined application category. Cytokine Kit, human, MACSPlex Cytotoxic T/NK Cell Kit, human and the MACSPlex Cytokine Kit, mouse cannot be mixed within a single experiment.

Overview of combinable reagents and necessary software versions to run Express Modes

Application category	Available target analytes	Standard	Basic Kit
Human Cytokine 12	IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α	MACSPlex Cytokine 12 Standard, human (130-106-197)	MACSPlex Cytokine Basic Kit, human and mouse (130-109-701)
Mouse Cytokine 10	IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α	MACSPlex Cytokine 10 Standard, mouse (130-106-198)	MACSPlex Cytokine Basic Kit, human and mouse (130-109-701)
Human Cytotoxic T/NK Cell	IL-2, IL-4, IL-6, IL-10, IL17A, IL-21, GM-CSF, Granzyme B, IFN-γ, MCP-1, TNF-α, Perforin	MACSPlex Mix Cytotoxic Standard (130-125-766)	MACSPlex Mix Cytotoxic Basic Kit (130-125-767)

Basic principle

Principle of of MACSPlex T/NK Cell Assays

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Samples containing unknown levels of analytes are incubated with the antibody-coated MACSPlex Capture Beads, and analytes bind to the specific antibody. Two detection reagents, composed of a cocktail of either biotin-conjugated or APC-conjugated antibodies specific for the analytes, are added. Consequently, sandwich complexes are formed between the MACSPlex Capture Beads, the analyte and the Detection Reagent Biotin while the Detection Reagent APC binds to the biotinylated detection antibodies. These complexes can be analyzed based on the fluorescence characteristics.

Standards of known quantities of given analytes are provided with the kit and are used for the quantification of the analytes within the unknown samples.

Prerequisites

For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.

Analysis with MACSQuant Analyzers and Express Modes

Exemplary analysis and results using a MACSPlex Cytokine Kit

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When using the MACSQuant Analyzer 10 or MACSQuant X, our MACSplex Kits can be combined with fully automated Express Modes.

By this, an automated gating strategy to populations of interest will be applied and the gates will be automatically adjusted for each data file individually to achieve optimal results.

Furthermore, a one-click batch analysis of all samples belonging to one experiment can be performed to directly compare your data. The concentrations of each analyte will be presented as an exportale table as well as a ready-to-publish graph.

ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.

Analysis with other instruments

For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays.

30-day trial versions are available for both Flowlogic Software and the Beadlogic Add-on.

Additionally, third-party software can be used for analysis.