Clone REAL137 is an antibody fragment derived from the full CD8a antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL137 is specific for the mouse CD8a antigen, also known as LY-2 or T8, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ
+ cells. CD8a is further detected on most thymocytes (CD4
+CD8a
+ and CD4
–CD8
+ thymocytes). Cytotoxic T cells and thymocytes express CD8a as heterodimer with CD8b, whereas dendritic cells and TCRγ/δ
+ cells express a CD8a/CD8a homodimer. It mediates efficient cell-cell interactions within the immune system.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.
