Clone:
REA304
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IHC, IF
Alternative names:
B-cell activation protein, Cell surface protein HB15

Extended validation for CD83 Antibody, anti-mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD83. Mouse splenocytes were stimulated with 100ng/mL LPS for 3 days at 37°C 5% CO2 and were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD83. Mouse splenocytes were stimulated with 100ng/mL LPS for 3 days at 37°C 5% CO2 and were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD83. Mouse splenocytes were stimulated with 100ng/mL LPS for 3 days at 37°C 5% CO2 and were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD83. Mouse splenocytes were stimulated with 100ng/mL LPS for 3 days at 37°C 5% CO2 and were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD83. Mouse splenocytes were stimulated with 100ng/mL LPS for 3 days at 37°C 5% CO2 and were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD83 (REA304). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD83 (REA304). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD83 (REA304). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD83 Antibody, anti-mouse, REAfinity™

Overview

Clone REA304 recognizes the mouse CD83 antigen, a Single-pass type I membrane protein which belongs to the immunoglobulin superfamily. Murine CD83 is moderately expressed on resting T cells and DC, but strongly increases in its expression on T cells following activation with antigenic peptides or T cell receptor-specific monoclonal antibody. When returning to the resting state, T cells down-regulate CD83 again. Histological analysis of serial spleen sections revealed a CD83 expression pattern resembling that of MIDC-8, a known murine DC marker molecule.
Additional information: Clone REA304 displays negligible binding to Fc receptors.

Alternative names

B-cell activation protein, Cell surface protein HB15

Detailed product information

Technical specifications

CloneREA304
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD83
Alternative names of antigenB-cell activation protein, Cell surface protein HB15
Molecular mass of antigen [kDa]19
Distribution of antigendendritic cells, T cells, spleen
Entrez Gene ID12522
RRIDAB_2659307, AB_2659308, AB_2659309, AB_2659310, AB_2659311, AB_2659312, AB_2659313, AB_2659314, AB_2659315, AB_2659316, AB_2659317, AB_2659306

Resources for CD83 Antibody, anti-mouse, REAfinity™

Certificates

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References for CD83 Antibody, anti-mouse, REAfinity™

Publications

  1. Lüthje, K. et al. (2008) CD83 regulates splenic B cell maturation and peripheral B cell homeostasis. Int. Immunol. 20(8): 949-960
  2. Twist, C. J. et al. (1998) The mouse CD83 gene: structure, domain organization, and chromosome localization. Immunogenetics 48(6): 383-393
  3. Wolenski, M. et al. (2003) Expression of CD83 in the murine immune system. Med. Microbiol. Immun. 192(4): 189-192

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