Clone:
REA220
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
Integrin-associated protein, IAP, MER6, OA3, CD47

Extended validation for CD47 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
View details
Overlay histogram showing flow cytometric analysis of CD47 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD47-PE (REA220). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD47 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD47-PE (REA220). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD47 Antibody, anti-human, REAfinity™

Overview

Clone REA220 recognizes CD47, a heavily glycosylated protein with an extracellular IgV domain, five transmembrane domains, and a cytoplasmic tail, which exists in four different isoforms. CD47 interact with integrins, thrombospondin-1 (TSP-1), and cell surface gylcoproteins SIRPα and SIRPβ. Owing to these interactions, CD47 plays an important role in lymphocyte homeostasis, maturation and activation of dendritic cells, cellular transmigration, and phagocytosis of cells. Expression of CD47 is found ubiquitously.
Additional information: Clone REA220 displays negligible binding to Fc receptors.

Alternative names

Integrin-associated protein, IAP, MER6, OA3, CD47

Detailed product information

Technical specifications

CloneREA220
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD47
Alternative names of antigenIntegrin-associated protein, IAP, MER6, OA3, CD47
Distribution of antigenB cells, endothelial cells, epithelial cells, T cells
RRIDAB_2819555, AB_2819520, AB_2802033, AB_2802017, AB_2658396, AB_2658397, AB_2658402, AB_2658403, AB_2658404, AB_2658405, AB_2658408, AB_2658409, AB_2658410, AB_2658411, AB_2658412, AB_2658413

References for CD47 Antibody, anti-human, REAfinity™

Publications

  1. Demeure, C. E. et al. (2000) CD47 engagement inhibits cytokine production and maturation of human dendritic cells. J. Immunol. 164: 2193-2199
  2. Burger, P. et al. (2012) CD47 functions as a molecular switch for erythrocyte phagocytosis. Blood 119: 5512-5521
  3. Kusakari, S. et al. (2008) Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47–SHPS-1 system. J. Cell. Sci. 121: 1213-1223

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