Clone:
REA567
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC

Extended validation for Basophils Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Basophils. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Basophils antibodies. As a control, Basophils antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Basophils. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Basophils antibodies. As a control, Basophils antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Basophils. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Basophils antibodies. As a control, Basophils antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Basophils. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Basophils antibodies. As a control, Basophils antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Basophils Antibody, anti-human, REAfinity™

Overview

Clone REA567 recognizes the population of human basophils. Basophils appear in many specific kinds of inflammatory reactions, particularly those that cause allergic symptoms, like parasitic infections and allergies. They secrete a wide variety of proinflammatory agents upon activation such as histamine and cytokines.
Additional information: Clone REA567 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA567
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenBasophils
Distribution of antigenbasophils
RRIDAB_2819432, AB_2651254, AB_2651255, AB_2651256, AB_2651257, AB_2819433

Resources for Basophils Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for Basophils Antibody, anti-human, REAfinity™

Publications

  1. Schroeder, J. T. (2009) Basophils beyond effector cells of allergic inflammation. Adv. Immunol. 101: 123-161
  2. Nakanishi, K. (2010) Basophils as APC in Th2 response in allergic inflammation and parasite infection. Curr. Opin. Immunol. 22(6): 814-820

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