The B Cell Isolation Kit was developed for the fast and efficient isolation untouched resting B cells from single-cell suspensions of lymphoid tissues. The separation protocol is faster now! Download the updated datasheet.

Data and images for B Cell Isolation Kit, mouse

Figures

Figure 1

Isolation of untouched B cells from a mouse spleen single-cell suspension using the B Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column.
Unseparated fraction
Isolated CD45R
+
B cells
View details

Figure 1

Isolation of untouched B cells from a mouse spleen single-cell suspension using the B Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column.
View details

Figure 1

Isolation of untouched B cells from a mouse spleen single-cell suspension using the B Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column.

Specifications for B Cell Isolation Kit, mouse

Overview

The B Cell Isolation Kit was developed for the fast and efficient isolation untouched resting B cells from single-cell suspensions of lymphoid tissues. The separation protocol is faster now! Download the updated datasheet.

Detailed product information

Detailed separation procedure

Highly pure resting B cells are isolated by magnetic labeling and depletion of CD43-expressing B cells (activated B cells, plasma cells and CD5
+
B-1a cells) and non-B cells. CD43
+
B cells, T cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells are labeled with a cocktail of biotin-conjugated antibodies against CD43 (Ly-48), CD4 (L3T4), and Ter-119, as well as Anti-Biotin MicroBeads. The B Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-target cells and Anti-Biotin MicroBeads.

Downstream applications

Untouched B cells may be used for further magnetic separation of B cells into subpopulations. B cells isolated with the B Cell Isolation Kit were e.g. analyzed for transgene expression by Northern blot analysis in a transgenic reporter model.
1

Columns

MS, LS, or autoMACS
®
Columns.

Resources for B Cell Isolation Kit, mouse

References for B Cell Isolation Kit, mouse

Publications

  1. Harrow, F. and Ortiz, B. D. (2005) The TCRalpha locus control region specifies thymic, but not peripheral, patterns of TCRalpha gene expression. J. Immunol. 175: 6659-6667

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