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|Principle||Any positive and untouched isolation can be performed to separate target cells from PBMCs, cultured cells, and dissociated tissue. In addition, cells can be directly isolated from whole blood or bone marrow using StraightFrom® Whole Blood MicroBeads. Overall more than 400 different MicroBeads and Isolation Kits are available and we are steadily expanding our portfolio. Upon automated separation, labeled cells as well as non-labeled cells are obtained and can be used for a variety of downstream applications such as functional and molecular assays, chimerism analysis, and flow cytometry. To ensure cell viability and integrity, cells are kept cooled in Chill Racks.|
|Monitoring||Pre-set programs for all MACS® MicroBeads and Isolation Kits ensure accurate sample handling. Sensors constantly monitor the status of the autoMACS® Pro, buffer levels, and column status. This ensures optimal instrument performance and safety of samples and operators.|
|Level of automation||Fully automated|
|Footprint||Without MiniSampler: 605x343 mm (W x D)|
With MiniSampler: 605x455 mm (W x D)
Height: 392.5-454 mm (adjustable touchscreen)
It is a benchtop-sized instrument that will easily fit under a common laminar flow hood.
|Characteristics||The reusable autoMACS column is specifically designed for use with the autoMACS Pro Separator delivering high cell purities and recoveries. The column is compatible with all starting materials and available MicroBeads and Isolation Kits.|
One or two columns can be installed and thus increase the flexibility with regards to high recovery or high purity processes. Once installed, the columns become part of a closed fluidic system that keeps you safe when dealing with hazardous samples. Monitoring and priming of the column are automatically managed by the instrument software.
|Capacity||- Up to 4 x 109 total cells/sample|
- Up to 2 x 108 magnetically labeled cells/sample
- Up to 15 mL of whole blood
|Sample type||- PBMCs|
- Whole blood
- Bone marrow
- Single-cell suspensions from dissociated tissue or tumor
- Cultured cells (cell lines and primary cells)
|Species||- Human |
- Non-human primate
- any others are possible with indirect MicroBeads
|Number of samples||- 6 samples with 5 mL tubes|
- 5 samples with 15 mL tubes
- 3 samples with 50 mL tubes
|Volume||0.2 mL - 50 mL sample volume depending on MicroBeads used|
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