The MACSprep™ Forensic Sperm MicroBead Kit, human has been developed for the positive selection of human sperms from cell mixture obtained from forensic samples.

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Workflow for the positive selection of human sperms

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Workflow for the positive selection of human sperms

Specifications for MACSprep™ Forensic Sperm MicroBead Kit, human

Overview

The MACSprep™ Forensic Sperm MicroBead Kit, human has been developed for the positive selection of human sperms from cell mixture obtained from forensic samples.

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Background information

The MACSprep™ Forensic Sperm MicroBead Kit, human has been developed for the fast isolation of sperm cells from standard proteinase K treated samples without performing differential lysis. After proteinase K treatment, the free DNA is removed and the isolation of sperm cells is performed with magnetic separation. Sperm cells are magnetically labeled with Anti-Spermatozoa MicroBeads, which recognizes a specific antigen on mature sperm head. Then, the cell suspension is loaded onto a MACS
®
Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled sperm cells are retained within the column. The unlabeled non-sperm cells run through; this cell fraction contains epithelial cells. After removing the column from the magnetic field, the magnetically retained sperm cells can be eluted as the positively selected cell fraction.

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