autoMACS^® Pro Separator

Separation strategies

Starting material

Consumables

• autoMACS Columns
• autoMACS Running Buffer - MACS Separation Buffer 
• autoMACS Washing Solution
• autoMACS Rinsing Solution

Accessories

• Chill Racks:  Chill 5 Rack, Chill 15 Rack, Chill 50 Rack
• Chill Rack Set:  5, 15, 50 Rack Set
• autoMACS Pro Angle Connector Set
• autoMACS Pro Protection Cover
• autoMACS Pro Laminar Hood Plate 
• MACS Reagent Rack 4
• autoMACS Pro Autolabeling Upgrade Kit

• autoMACS Pro Separator User Manual
• autoMACS Pro Separator short instructions

Brochures and posters

• autoMACS Pro Separator - The gold standard in automated cell separation

Scientific posters

• Gene expression analysis reveals mechanisms with potential relevance to NK cell expansion protocols for immunotherapy
• Novel methods for the isolation of tumor cells from human, mouse, and xenografted tumors
• T helper cells a complete workflow for cell preparation, isolation, stimulation, polarization, and analysis
• High-purity enrichment of human hematopoietic stem cells utilizing MACS® Technology
• An in vitro method for the identification of DNP- and TNP-specific T cells in the naive compartment of human blood
• Flow cytometric multicolor NK cell phenotyping as a potential tool for donor selection and NK cell reconstitution analysis
• Workflow automation and parallelization improves the isolation and analysis of tumor-infiltrating immune cell subpopulations
• Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol. Zhang, H. et al. SfN (2016)
• Gene expression analysis of CD14⁺ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements

Customer reports and application notes

• Isolation of monocytes with high purity directly from whole blood for transcriptome analysis in translational research. Foucher et al. (2013) MACS&more 15(1)
• CD56+ cell depletion from human PBMCs applied to validate an HIV-specific ADCC mechanism. Bardhi. A. (2017)
• Effector memory T helper cells secrete IFN-γ upon stimulation with cytokines: a role in chronic inflammation. Sattler et al. (2011) MACS&more 13(1).
• An automated method for purification of CD138+ cells from whole bone marrow samples for multiple myeloma research. Mossafa and Defasque (2013) MACS&more 15(1)
• MACS® Technology facilitates mixed lymphocyte reaction
• Measuring prolyl aminopeptidase activity in extracts prepared from magnetically purified malaria parasites. da Silva et al. (2013) MACS&more 15(1).
• Willasch et al. (2012) Enrichment of cell subpopulations by MACS® Technology for PCR-based chimerism analysis. MACS&more 13(1).
• Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflows. Herron, T. (2017)
• autoMACS Pro vs. FACS
• CCR4 – a potential marker for the selective removal of effector-type FOXP3+CD4+ regulatory T cells in cancer immunotherapies. Sugiyama & Nishikawa (2018)
• autoMACS Pro and Multiple Myeloma Research
• Allogenic T cell infusion alters iron regulatory gene expression. Deeg, H.J. (2018)
• Modulation of allergic airway inflammation by the histamine H4 receptor: analysis by flow cytometry. Hartwig et al. (2012) MACS&more 14(1).
• Purification of YSD antibody libraries using the autoMACS® Pro Separator. Miller K.D. et al. (2012)
• Generation of functional CAR T cells from pan T cells isolated by MACS® technology
• Generation of functional CAR T cellsfrom CD4+ and CD8+ T cells
• Mouse NK cells isolated to high purity by MACS® Technology are fully functional. Meinhardt et al. (2013) MACS&more 15(1).
• Best practices in optimizing sample processing for improved flow cytometry. Spring C. (2017)
• Purification of human hematopoietic cells from xenografts for the assessment of functional and genomic identity. Dobson, S. et al. (2015)
• TCR clonotype sequence is only one factor defining the antiviral capacity of HIV-specific CD8+ T cells. Flerin, N. (2018)
• Depletion of mouse cells from dissociated patient derived xenograft tumors using the gentleMACS™ OctoDissociator with Heaters, followed by magnetic cell separation. Moro, M. (2014)

Other resources

• autoMACS Pro Separator autolabeling list

Automated cell separation for downstream omics applications (reference list)

Publications

1. Ferretti, A. et al. (2020) Unbiased Screens Show CD8⁺ T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein. Immunity. 53(5): 1095-1107
2. Saini S. et al. (2020) Human endogenous retroviruses form a reservoir of T cell targets in hematological cancers. Nat Commun. 11:5660
3. Akio T. et al. (2020) Activated invariant natural killer T cells directly recognize leukemia cells in a CD1d-independent manner. Cancer Sci. 111(7): 2223-2233
4. Gabr H. et al. (2020) CRISPR-mediated gene modification of hematopoietic stem cells with beta-thalassemia IVS-1-110 mutation. Stem Cell Res Ther. 11: 390
5. Hasegawa, N. et al. (2019) Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter. Sci Rep 9: 20047
6. Kulis, M. et al. (2015) Whole‑genome fingerprint of the DNA methylome during human B cell differentiation. Nat. Genet. 47(7): 746-756
7. Haus, D. L. et al. (2014) CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice Stem Cell Res. 13(2): 214-226
8. Kim, MS. et al. (2014) A draft map of the human proteome. Nature 509(7502): 575-581
9. Kuwahara, M. et al. (2014) The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis Nat Commun 2(5): 3555
10. Riquelme, P. et al. (2013) IFN-γ-induced iNOS expression in mouse regulatory macrophages prolongs allograft survival in fully immunocompetent recipients Mol. Ther. 21(2): 409-422
11. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076