autoMACS
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Pro Separator - Starter Kit

Name: autoMACS® Pro Separator - Starter Kit
Availability: InStock

autoMACS
^®
Pro Separator - Starter Kit

Product overview

The autoMACS

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Pro Separator is a benchtop instrument for high-speed magnetic cell sorting of multiple samples. Employing MACS

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autoMACS
^®
Pro Separator - Starter Kit

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autoMACS

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Pro Separator

autoMACS

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Pro Separator

Specifications for
autoMACS
^®
Pro Separator - Starter Kit

Overview

The autoMACS

^®

Pro Separator is a benchtop instrument for high-speed magnetic cell sorting of multiple samples. Employing MACS

^®

Technology, the autoMACS Pro Separator is designed for cell isolation in a fully automated, walk-away fashion.

Detailed product information

The autoMACS Pro Separator combines all the benefits of the proven autoMACS Technology with novel features such as automated magnetic labeling and sensor-controlled processing of multiple samples. The instrument status can be viewed from a distance, facilitated by illumination of buffer bottles by light-emitting diodes. The instrument is operated via touchscreen and intuitive screen menus. At the touch of a button, multisample labeling and cell separation are performed, minimizing hands-on time. Sample integrity is also maintained by use of MACS Chill Racks that allow for cooling of samples and eluted fractions. With its compact size, the autoMACS Pro Separator easily fits into a standard laminar flow hood or safety cabinet.

Applications

The autoMACS Pro Separator is compatible with more than 250 MACS Cell Separation Reagents for easy and fast isolation of virtually any cell type from any species.

Furthermore, the autoMACS Pro Separator enables cell sorting directly from whole blood or bone marrow without the need for density-gradient centrifugation or erythrocyte lysis.

The compatibility of MACS Technology with flow cytometry allows the use of the autoMACS Pro Separator as an alternative to flow sorting. Alternatively, the autoMACS Pro Separator can be used as a pre-sorter for speeding up flow sorting.

Resources for
autoMACS
^®
Pro Separator - Starter Kit

Documents and Protocols

Brochures/posters

autoMACS Pro Separator - The gold standard inautomated cell separation

Scientific posters

Gene expression analysis reveals mechanisms with potential relevance to NK cell expansion protocols for immunotherapy

Scientific posters

Novel methods for the isolation of tumor cells from human, mouse, and xenografted tumors

Scientific posters

T helper cells a complete workflow for cell preparation, isolation, stimulation, polarization, and analysis

Scientific posters

High-purity enrichment of human hematopoietic stem cells utilizing MACS® Technology

Scientific posters

An in vitro method for the identification of DNP- and TNP-specific T cells in the naive compartment of human blood

Scientific posters

Flow cytometric multicolor NK cell phenotyping as a potential tool for donor selection and NK cell reconstitution analysis

Scientific posters

Fenofibrate treatment modulates gene expression activity in peripheral blood monocytes of healthy volunteers

Scientific posters

Workflow automation and parallelization improves the isolation and analysis of tumor-infiltrating immune cell subpopulations

Scientific posters

Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol. Zhang, H. et al. SfN (2016)

Scientific posters

Gene expression analysis of CD14+ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements

App notes/customer reports

Isolation of monocytes with high purity directly from whole blood for transcriptome analysis in translational research. Foucher et al. (2013) MACS&more 15(1).

App notes/customer reports

CD56+ cell depletion from human PBMCs applied to validate an HIV-specific ADCC mechanism. Bardhi. A. (2017)

App notes/customer reports

Effector memory T helper cells secrete IFN-γ upon stimulation with cytokines: a role in chronic inflammation. Sattler et al. (2011) MACS&more 13(1).

App notes/customer reports

An automated method for purification of CD138+ cells from whole bone marrow samples for multiple myeloma research. Mossafa and Defasque (2013) MACS&more 15(1)

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MACS® Technology facilitates mixed lymphocyte reaction

App notes/customer reports

Measuring prolyl aminopeptidase activity in extracts prepared from magnetically purified malaria parasites. da Silva et al. (2013) MACS&more 15(1).

App notes/customer reports

Willasch et al. (2012) Enrichment of cell subpopulations by MACS® Technology for PCR-based chimerism analysis. MACS&more 13(1).

App notes/customer reports

Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflows. Herron, T. (2017)

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autoMACS Pro vs. FACS

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CCR4 – a potential marker for the selective removal of effector-type FOXP3+CD4+ regulatory T cells in cancer immunotherapies. Sugiyama & Nishikawa (2018)

App notes/customer reports

autoMACS Pro and Multiple Myeloma Research

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Allogenic T cell infusion alters iron regulatory gene expression. Deeg, H.J. (2018)

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Modulation of allergic airway inflammation by the histamine H4 receptor: analysis by flow cytometry. Hartwig et al. (2012) MACS&more 14(1).

App notes/customer reports

Purification of YSD antibody libraries using the autoMACS® Pro Separator. Miller K.D. et al. (2012)

App notes/customer reports

Generation of functional CAR T cells from pan T cells isolated by MACS® technology

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Generation of functional CAR T cellsfrom CD4+ and CD8+ T cells

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Mouse NK cells isolated to high purity by MACS® Technology are fully functional. Meinhardt et al. (2013) MACS&more 15(1).

App notes/customer reports

Best practices in optimizing sample processing for improved flow cytometry. Spring C. (2017)

App notes/customer reports

Purification of human hematopoietic cells from xenografts for the assessment of functional and genomic identity. Dobson, S. et al. (2015)

App notes/customer reports

TCR clonotype sequence is only one factor defining the antiviral capacity of HIV-specific CD8+ T cells. Flerin, N. (2018)

App notes/customer reports

Depletion of mouse cells from dissociated patient derived xenograft tumors using the gentleMACS™ OctoDissociator with Heaters, followed by magnetic cell separation. Moro, M. (2014)

References

autoMACS Pro Separator - selected references

Other downloads

autoMACS® Pro Separator autolabeling list

Other downloads

autoMACS Pro Separator short instructions

Reviews for
autoMACS
^®
Pro Separator - Starter Kit

AutoMACS Pro Separator from Miltenyi Biotec

autoMACS Pro Separator - Starter Kit (130-092-545)

The autoMACS Pro splits the sample into negative and positive fractions of the defined volume, with good purity and fine cell viability.

Automated Cell Separator

autoMACS Pro Separator - Starter Kit (130-092-545)

Our lab routinely separated different cell populations from human PBMCs (CD8, CD4, CD56...).

AutoMACS Separator

autoMACS Pro Separator - Starter Kit (130-092-545)

Our Laboratory often needs to purify specific cell types in order to infect with HIV, or to engineer immune effector cells such as NK cells and CD8 T cells for enhanced anti-HIV activity. We use the bead-based magnetic separation and the autoMACS machine for this purpose.

Easy to Use Automated Magnetic Cell Sorting

autoMACS Pro Separator - Starter Kit (130-092-545)

The autoMACS Pro Separator from Miltenyi Biotec allows full automation of magnetic cell sorting of your desired cell population of interest for your research. It has the capability to isolate cells from your purified heterogeneous cell sample, but also directly from primary tissue sources such as whole blood or bone marrow. If you are already experienced with MACS products for manual cell separation, the transition to automated cell sorting is straight forward as several of the reagents used by both techniques are the same. MACS Microbead technology in combination with the autoMACS Pro Separator offers an alternative or complement to flow cytometry based sorting, and is compatible with several downstream applications including but not limited to cell culture, functional assays, flow cytometry, PCR, and large scale gene expression analysis. Our lab routinely used the autoMACS separator to obtain highly pure immune subsets from peripheral blood mononuclear cells.

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References for
autoMACS
^®
Pro Separator - Starter Kit

Publications

Carlson, S. et al. (2011) Cardiac mesenchymal stem cells contribute to scar formation after myocardial infarction Cardiovasc. Res. 91: 99-107
Kulis, M. et al. (2015) Whole‑genome fingerprint of the DNA methylome during human B cell differentiation. Nat. Genet. 47(7): 746-756
Haus, D. L. et al. (2014) CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice Stem Cell Res. 13(2): 214-226
Kim, MS. et al. (2014) A draft map of the human proteome. Nature 509(7502): 575-581
Kuwahara, M. et al. (2014) The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis Nat Commun 2(5): 3555
Riquelme, P. et al. (2013) IFN-γ-induced iNOS expression in mouse regulatory macrophages prolongs allograft survival in fully immunocompetent recipients Mol. Ther. 21(2): 409-422
Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076
Liu, Y. et al. (2011) Expression of p16INK4a prevents cancer and promotes aging in lymphocytes. Blood 117: 3257-3267
Perez, J. et al. (2011)
Accumulation of CD4
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T cells in the colon of CsA-treated mice following myeloablative conditioning and bone marrow transplantation
Am. J. Physiol. Gastrointest. Liver Physiol. 300: G843-G852
Vargova, K. et al. (2011) MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia. Blood 2011(117): 3816-3825
Willasch et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis Bone Marrow Transplant. 45: 181-189
Lindstrom D.L. and Gottschling D.E. (2009) The mother enrichment program: a genetic system for facile replicative life span analysis in Saccharomyces cerevisiae Genetics 183(2): 413-422

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