MBTP 38: Immunophenotyping of neonatal cardiomyocytes from rat using flow cytometry

Miltenyi Biotec-tested panel 38 (MBTP 38)

This application protocol describes the characterization of distinct neonatal cardiomyocyte (CM) subtypes (atrial and ventricular) from rat using flow cytometry. 

Protocol

Flow cytometry analysis of enriched neonatal rat CMs. Neonatal rat CMs were isolated from dissociated whole hearts of rats using the Neonatal Cardiomyocyte Isolation Kit, rat. The cells were labeled with Cardiac Troponin T Antibody-FITC, MLC2a Antibody-VioBlue, and MLC2v Antibody-APC after enrichment and analyzed by flow cytometry using the MACSQuant Analyzer 10. CM subtypes were characterized by differential expression of MLC2a (in atrial CMs, A and C) and MLC2v (in ventricular CMs, B and C).

Materials

Matching products: