Miltenyi Biotec-tested panel 33 (MBTP 33)
This application protocol describes the characterization of activated T cells within antigen-stimulated peripheral blood mononuclear cells (PBMCs) via simultaneous measurement of surface markers and intracellular cytokines using flow cytometry. Prior to antibody staining PBMCs were stimulated with a cocktail of PepTivator CMV pp65, PepTivator EBV consensus, and PepTivator AdV5 Hexon from Miltenyi Biotec.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
Viobility™ 405/452 Fixable Dye | - | Viability | - | 450/50 (violet) |
IFN-γ | REA600 | T cell activation | Vio® R720 | 725/40 (red) |
CD3 | REA613 | T cells | APC-Vio 770 | 785/62 (red) |
CD4 | REA623 | T helper cells | Vio Bright B515 | 525/50 (blue) |
CD8 | REA734 | Cytotoxic T cells | VioGreen™ | 525/50 (violet) |
TNF-α | REA656 | T cell activation | PE | 579/34 (blue) |
CD154 | REA238 | T cell activation | APC | 667/30 (red) |
CD14 | REA599 | Cell type exclusion | VioBlue® | 450/50 (violet) |
CD20 | REA780 | Cell type exclusion | VioBlue | 450/50 (violet) |
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