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Mouse spleen cells were either left unstimulated (left image) or stimulated with KLH for 6 hours. After 2 hours, brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-2-PE and CD154-APC. Cells were analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells.
Clone | JES6-5H4 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG2bκ |
Isotype control | Isotype Control Antibody, rat IgG2b |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | mouse |
Antigen | IL-2 |
Molecular mass of antigen [kDa] | 17 |
Entrez Gene ID | 16183 |
RRID | AB_2660009, AB_2660010, AB_10829301 |
Clone | JES6-5H4 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG2bκ |
Isotype control | Isotype Control Antibody, rat IgG2b |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | mouse |
Antigen | IL-2 |
Molecular mass of antigen [kDa] | 17 |
Entrez Gene ID | 16183 |
RRID | AB_2660009, AB_2660010, AB_10829301 |
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