IL-2 Antibody, anti-mouse

Name: IL-2 Antibody, anti-mouse
Availability: InStock

IL-2 Antibody, anti-mouseExtended Validation

Clone:

JES6-5H4

Type of antibody:

Primary antibodies, Functional-grade antibodies

Isotype:

rat IgG2bκ

Applications:

FA, ICFC, MICS, IF, IHC

Intracellular flow cytometry applications forIL-2 Antibody, anti-mouse

APC
PE

Conjugate:

APC

Recommended dilution:

1:10

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-102-387

Protocols:

Intracellular flow cytometry staining protocol (Inside Stain Kit 1:10, protocol B)

Description:

Mouse spleen cells were either left unstimulated (left image) or stimulated with KLH for 6 hours. After 2 hours, brefeldin A was added. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-2-PE and CD154-APC. Cells were analyzed by flow cytometry. Gating was performed according to CD4 expression and side scatter properties of the cells.

MICS (MACSima Imaging Cyclic Staining) applications forIL-2 Antibody, anti-mouse

APC
PE

Conjugate:

APC

Recommended dilution:

1:10

Fixation:

Acetone

Tested:

during development

Species tested:

mouse

Products used:

130-102-387

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Functional assay applications forIL-2 Antibody, anti-mouse

pure-functional grade

Conjugate:

pure-functional grade

Tested:

during development

Products used:

130-095-736

Type of functional assay:

neutralization of cytokine activity

Extended validation for IL-2 Antibody, anti-mouse

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for IL-2 Antibody, anti-mouse

Overview

Interleukin 2 (IL-2), mainly produced by activated CD4

⁺

T cells, is a central regulator of the immune response. It induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, monocytes. and oligodendrocytes. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance, and anti-inflammatory reactions.

Detailed product information

Technical specifications

Clone	JES6-5H4
Clonality	monoclonal
Isotype	rat IgG2bκ
Isotype control	Isotype Control Antibody, rat IgG2b
Host	rat
Type of antibody	Primary antibodies, Functional-grade antibodies
Species	mouse
Antigen	IL-2
Molecular mass of antigen [kDa]	17
Entrez Gene ID	16183
RRID	AB_2660009, AB_2660010, AB_10829301

Resources for IL-2 Antibody, anti-mouse

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

Applications

Extended validation